PXD051782-1

PXD051782 is an original dataset announced via ProteomeXchange.

Title	The DEAD-box RNA helicase PfDOZI imposes opposing actions on RNA metabolism in Plasmodium falciparum
Description	In malaria parasites, the regulation of mRNA translation, storage and degradation during development and life stage transitions remains largely unknown. Here, we functionally characterized the DEAD-box RNA helicase PfDOZI in P. falciparum. Disruption of pfdozi enhanced asexual proliferation but reduced sexual commitment and impaired gametocyte development. By quantitative transcriptomics, we show that PfDOZI is involved in the regulation of invasion-related genes and sexual stage-specific genes during different developmental stages. PfDOZI predominantly participates in processing body-like mRNPs in schizonts but germ cell granule-like mRNPs in gametocytes to impose opposing actions of degradation and protection on different mRNA targets. We further show the formation of stress granule-like mRNPs during nutritional deprivation, highlighting an essential role of PfDOZI-associated mRNPs in stress response. Here, we demonstrate that PfDOZI participates in distinct mRNPs to maintain mRNA homeostasis in response to life-stage transition and environmental changes by differentially executing post-transcriptional regulation on the target mRNAs.
HostingRepository	PRIDE
AnnounceDate	2024-04-30
AnnouncementXML	Submission_2024-04-30_06:35:21.720.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HUI MIN
SpeciesList	scientific name: Plasmodium falciparum MLW.2749; NCBI TaxID: 1226408;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-26 10:35:36	ID requested
⏵ 1	2024-04-30 06:35:22	announced
2	2024-10-22 06:37:49	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Plasmodium falciparum, Post-transcriptional regulation, Stress granule., PfDOZI, Processing body, RNA helicase

Liwang Cui
contact affiliation	Division of Infectious Diseases and International Medicine Department of Internal Medicine Morsani College of Medicine University of South Florida
contact email	liwangcui@usf.edu
lab head
HUI MIN
contact affiliation	University of South Florida
contact email	huim868@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051782
     1. Label: PRIDE project
     2. Name: The DEAD-box RNA helicase PfDOZI imposes opposing actions on RNA metabolism in Plasmodium falciparum