PXD051745-1

PXD051745 is an original dataset announced via ProteomeXchange.

Title	The Protein Composition of Human Adenovirus Replication Compartments.
Description	Human adenoviruses are double–stranded DNA viruses that replicate in the cell nucleus and induce the formation of replication compartments (RCs) that are critical in viral replication and control of virus-host interactions. RCs are specialized virus-induced subnuclear microenvironments in which viral genome replication and expression are orchestrated, but also where host proteins known to restrict viral replication are co-opted and subverted. However, the protein composition of these RCs remains largely unexplored. In this study, we isolated adenovirus RC-enriched fractions from infected cells at different times post-infection and employed a tandem mass tag-based quantitative mass spectrometry approach to identify proteins associated with RCs. To validate the RC-protein components identified by mass spectrometry, we employed immunofluorescence and western blotting techniques. Proteins previously described to colocalize in RCs in infected cells were identified in the isolated subnuclear fractions. Moreover, we were able to validate previously unidentified RC-associated proteins, including the High Mobility Group Box 1, the SET nuclear proto-oncogene, the Structure Specific Recognition Protein 1 and the CCCTC-binding protein. These findings reveal an elaborate network of host and viral proteins potentially relevant for RC formation and function. The results also suggest potential candidate targets for therapeutic intervention and further mechanistic insights into the molecular basis of virus-host interactions
HostingRepository	PRIDE
AnnounceDate	2024-11-20
AnnouncementXML	Submission_2024-11-20_07:49:10.214.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051745
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ramón A. González
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-25 14:40:31	ID requested
⏵ 1	2024-11-20 07:49:10	announced

10.1128/MBIO.02144-24;

10.6019/PXD051745;

submitter keyword: viral replication compartment (RC), HMGB1,Human adenovirus (HAdV), biomolecular condensates (BMCs), histone chaperones, tandem mass tag-based quantitative mass spectrometry

Ileana M.
contact affiliation	Department of Molecular Biology, Cristea Lab, Princeton University, USA
contact email	icristea@princeton.edu
lab head
Ramón A. González
contact affiliation	Universidad Autónoma del Estado de Morelos
contact email	rgonzalez@uaem.mx
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051745
     1. Label: PRIDE project
     2. Name: The Protein Composition of Human Adenovirus Replication Compartments.