PXD051737 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nanobodies as novel tools to monitor the mitochondrial fission factor Drp1 |
Description | In cells, mitochondria undergo constant fusion and fission. An essential factor for fission is the mammalian dynamin-related protein 1 (Drp1). Dysregulation of Drp1 has been linked to neurodegenerative diseases including Parkinson’s as well as cardiovascular diseases and cancer. Here, we developed nanobodies (Nbs) for proteomics, advanced microscopy and live cell imaging of Drp1. To specifically enrich endogenous Drp1 with interacting proteins for proteomics, we functionalized high-affinity Nbs as capture matrices. Considering the wide range of research applications, the presented Nb toolset will open up new possibilities for advanced functional studies of Drp1 in disease-relevant models. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-29 |
AnnouncementXML | Submission_2024-05-29_15:35:42.160.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Claudia Cavarischia Rega |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-25 06:56:42 | ID requested | |
⏵ 1 | 2024-05-29 15:35:42 | announced | |
Publication List
Keyword List
submitter keyword: pulldown, proteomics, mitochondrial fission, Drp1,Nanobodies |
Contact List
Ulrich Rothbauer |
contact affiliation | Pharmaceutical Biotechnology, Eberhard Karls University Tübingen, Germany |
contact email | ulrich.rothbauer@uni-tuebingen.de |
lab head | |
Claudia Cavarischia Rega |
contact affiliation | Proteome Center Tuebingen Interfaculty Institute for Cell Biology University of Tuebingen |
contact email | claudia.cavarischia-rega@uni-tuebingen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD051737 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051737
- Label: PRIDE project
- Name: Nanobodies as novel tools to monitor the mitochondrial fission factor Drp1