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PXD051717-1

PXD051717 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleElectrophoresis-Correlative Data-Independent Acquisition (Eco-DIA) Improves the Sensitivity of Mass Spectrometry for Limited Proteome Amounts
DescriptionIn this project, we developed electrophoresis-correlative (Eco) data-independent acquisition (DIA) mass spectrometry (MS). We recognized a correlation between the measured mass-to-charge (m/z) and migration time (MT) for peptides during capillary electrophoresis (CE) electrospray ionization (ESI) MS. We leveraged this relationship to dynamically tailor the experimental settings of DIA on an orbitrap mass spectrometer. This approach substantially improved utilization of the limited duty cycle during tandem MS and detection sensitivity compared to the classical DIA. From 1 ng of the standard HeLa proteome digest, Eco-DIA identified ~38% more proteins than conventional DIA, without the assistance of a project-specific spectral library. Proteins that were quantified exclusively by Eco-DIA were in the lower abundance range. Eco-DIA improved the sensitivity of detection from limited amounts of proteomes using CE-ESI-MS.
HostingRepositoryPRIDE
AnnounceDate2024-09-17
AnnouncementXMLSubmission_2024-09-17_13:00:49.235.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPeter Nemes
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-24 14:00:48ID requested
12024-09-17 13:00:49announced
22024-10-22 06:58:59announced2024-10-22: Updated project metadata.
Publication List
10.1021/ACS.ANALCHEM.4C02330;
Keyword List
submitter keyword: proteomics, mass spectrometry,Single cell, orbitrap, capillary electrophoresis, data-independent acquisition
Contact List
Peter Nemes
contact affiliationDepartment of Chemistry & Biochemistry, University of Maryland at College Park
contact emailnemes@umd.edu
lab head
Peter Nemes
contact affiliationUniversity of Maryland, College Park
contact emailnemes@umd.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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