PXD051693-1

PXD051693 is an original dataset announced via ProteomeXchange.

Title	Single electron transfer between sulfonium and tryptophan enables site-selective photo crosslinking of methyllysine reader proteins
Description	Reader proteins play significant roles in cell signaling from site-specific lysine methylation, but progress of new reader investigations is rather slow. Photoreactive crosslinkers enable the discovery of reader proteins, but the chemical synthesis required to optimize the placement of the photoreactive group is laborious. In addition, active intermediates such as nitrene and carbene may cause significant non-specific crosslinking. Here we report dimethylsulfonium as a methyllysine mimic that binds to specific readers and can subsequently crosslink to a conserved tryptophan inside the binding pocket through single electron transfer under UV irradiation. The crosslinking requires σ-π electron-donor-acceptor interaction between sulfonium and indole as a key step, facilitating excellent tryptophan site-selectivity and orthogonality to distinct methyllysine readers. Moreover, excited tryptophan without proximate sulfonium leads to minimal non-specific crosslinking from relaxation. This method could escalate the discovery of methyllysine readers from complex cell samples. Furthermore, this photo crosslinking concept could also be expanded to develop more types of microenvironment-dependent conjugations to site-specific tryptophan.
HostingRepository	PRIDE
AnnounceDate	2024-06-30
AnnouncementXML	Submission_2024-06-30_01:59:49.577.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingyun Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-24 01:55:50	ID requested
⏵ 1	2024-06-30 01:59:49	announced
2	2024-10-22 06:47:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Methyllysine readers, sulfonium, tryptophan, photo crosslinking

Mingxuan Wu
contact affiliation	1. Department of Chemistry, School of Science, Westlake University, Hangzhou 310030, Zhejiang Province, China 2. Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, Zhejiang Province, China 3. Institute of Natural Sciences, Westlake Institute for Advanced Study, Hangzhou 310024, Zhejiang Province, China
contact email	wumingxuan@westlake.edu.cn
lab head
Qingyun Yang
contact affiliation	Westlake University
contact email	yangqingyun@westlake.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD051693

PRIDE project URI

• PRIDE
  1. PXD051693
     1. Label: PRIDE project
     2. Name: Single electron transfer between sulfonium and tryptophan enables site-selective photo crosslinking of methyllysine reader proteins