PXD051662-1

PXD051662 is an original dataset announced via ProteomeXchange.

Title	Cytoplasmic mRNA decay controlling inflammatory gene expression is determined by pre-mRNA fate decision
Description	The fidelity of immune responses depends on a timely controlled and selective mRNA degradation that is largely driven by RNA-binding proteins (RBPs). It remains unclear whether the selection of an individual mRNA molecule for degradation is governed by stochastic or directed processes. Here, we show that tristetraprolin (TTP, also known as ZFP36), an essential anti-inflammatory RBP, destabilizes target mRNAs via a hierarchical molecular assembly. The formation of the assembly strictly relies on the interaction of TTP with RNA. The TTP homolog ZFP36L1 exhibits similar requirements, indicating a broader relevance of this regulatory program. Unexpectedly, the assembly of the cytoplasmic mRNA-destabilization complex is licensed in the nucleus by TTP binding to pre-mRNA, while cytoplasmic mRNA does not constitute a de novo TTP target. Hence, the fate of an inflammation-induced mRNA is decided concomitantly with its synthesis. This mechanism prevents the translation of excessive and potentially harmful inflammation mediators, irrespective of transcription.
HostingRepository	PRIDE
AnnounceDate	2025-01-07
AnnouncementXML	Submission_2025-01-07_05:44:51.254.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiQiang Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-23 06:12:56	ID requested
⏵ 1	2025-01-07 05:44:51	announced

Dataset with its publication pending

submitter keyword: mRNA decay, pre-mRNA binding, resolution of inflammation,Inflammation, TTP

Pavel Kovarik
contact affiliation	Max Perutz Labs, Campus Vienna Biocenter 5, 1030 Vienna
contact email	pavel.kovarik@univie.ac.at
lab head
WeiQiang Chen
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH
contact email	chenweiqiang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD051662

PRIDE project URI

• PRIDE
  1. PXD051662
     1. Label: PRIDE project
     2. Name: Cytoplasmic mRNA decay controlling inflammatory gene expression is determined by pre-mRNA fate decision