PXD051657-1

PXD051657 is an original dataset announced via ProteomeXchange.

Title	The human IRE1 in situ proximal signalosome
Description	The unfolded protein response (UPR) is a key adaptive pathway that controls endoplasmic reticulum (ER) homeostasis. The UPR is transduced by three ER-resident proteins acting as sensors of ER homeostasis in the lumen of this compartment and triggering select downstream signaling pathways in the cytosol and nucleus. Among them, IRE1α (referred to as IRE1 hereafter), a type I transmembrane protein, senses accumulation of improperly folded proteins in the ER lumen and transduces signals through both kinase and endoribonuclease activities in the cytosol. This mainly results in XBP1 mRNA unconventional splicing and RNA degradation (Regulated IRE1 Dependent Decay). Recently studies have also reported that IRE1-dependent protein-protein interactions (PPi) could modulate signaling, and drive additional non-canonical IRE1 functions. In the present study, we define the IRE1 signalosome based on literature and experimental approaches, as IRE1 binding partners able to alter IRE1 signaling. In particular, we explore the IRE1 in situ interactome using BioID linking the binding of direct IRE1 interacting partners to the regulation of IRE1 catalytic activity and identify novel functional connections with molecular machines involved in retrograde transport or RNA metabolism. This allows us to hypothesize on potentially novel IRE1 functions based on the nature of its interactome and its localization.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:13:35.409.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Negroni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-23 01:26:25	ID requested
⏵ 1	2026-09-06 16:13:36	announced

10.1038/s41467-026-73942-z;

Issaoui H, Zawil L, Bellone L, Goncalves D, Paul R, Di Mascio L, N, ú, ñ, ez-V, á, zquez S, Marchetti S, Chiche J, Nottet N, Le Goupil S, Laprade H, Bansard L, Hong Y, Wall R, Tejpar S, Truntzer C, Ghiringhelli F, Poudel S, Beere HM, Green DR, Pannequin J, Chevet E, Ricci JE, The mannosyltransferase DPM1 regulates the activity of ER-stress sensor IRE1 in colorectal cancer. Nat Commun, 17(1):(2026) [pubmed]

submitter keyword: Human, IRE1, UPR, BioID

Eric Chevet
contact affiliation	NSERM U1242, University of Rennes, Centre de Lutte contre le cancer Eugène Marquis
contact email	eric.chevet1@gmail.com
lab head
Luc Negroni
contact affiliation	CNRS
contact email	luc.negroni@igbmc.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD051657

PRIDE project URI

• PRIDE
  1. PXD051657
     1. Label: PRIDE project
     2. Name: The human IRE1 in situ proximal signalosome