PXD051654-1

PXD051654 is an original dataset announced via ProteomeXchange.

Title	Protein thermal stability-based characterization of dsRNA binding proteins
Description	Double-stranded RNA (dsRNA) binding proteins play key roles in numerous biological activities in cells, including innate immunity, RNA interference, and mRNA transport. Here, we established an analytical strategy to characterize dsRNA binding proteins from naive cell lysate by segmented proteome integral solubilityalteration assay combined with label free quantitative mass spectrometry. In this approach, we identified 218 dsRNA binding proteins from naive cell lysate of Hela cell. The data confirmed 18 known dsRBPs and identified 200 novel dsRBPs which have never been reported before.
HostingRepository	iProX
AnnounceDate	2024-04-23
AnnouncementXML	Submission_2025-03-05_18:40:47.278.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiang Na
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-22 23:42:02	ID requested
⏵ 1	2025-03-05 18:40:47	announced
2	2025-03-06 19:35:12	announced	2025-03-07: Update information.

Dataset with its publication pending

submitter keyword: dsRNA, dsRBP, thermal stability, Proteome Integral Solubility Alteration, innate immunity, data independent acquisition, mass spectrometry

Ruibing Chen
contact affiliation	Tianjin University
contact email	rbchen@tju.edu.cn
lab head
Jiang Na
contact affiliation	Tianjin University
contact email	16622080827@163.com
dataset submitter

iProX dataset URI