PXD051642-1

PXD051642 is an original dataset announced via ProteomeXchange.

Title	High-Throughput Part Characterization and Tool Development to Advance Chloroplast Synthetic Biology
Description	Plant synthetic biology holds promise for developing climate-resilient crop varieties particularly through advancements in engineering the chloroplast genome. However, the field faces limitations due to the scarcity of genetic tools and the long generation times of photosynthetic eukaryotes. To address these challenges, we established Chlamydomonas reinhardtii as a prototyping chassis for chloroplast synthetic biology, by significantly advancing plastome engineering tools. We developed an automation workflow that enabled the generation, handling, and analysis of 3,156 transplastomic Chlamydomonas strains and expanded the repertoire of selection markers for chloroplast transformation in Chlamydomonas to five, alongside establishing six new reporter genes. Moreover, we characterized over 140 regulatory parts, including promoters, UTRs, and intercistronic expression elements, all integrated within the Phytobrick cloning framework, for which we demonstrated a broad range of gene expression strength. Additionally, we explored library-based approaches to overcome the throughput limitations of biolistic chloroplast transformation. Finally, we demonstrated the utility of all tools by introducing a chloroplast-based synthetic photorespiration pathway. The high conservation of the chloroplast genetic system suggests that our findings in Chlamydomonas could be translated to advance chloroplast engineering in land plants and provides a prototyping platform for future crop improvements to address the challenges posed by a changing global climate.
HostingRepository	PRIDE
AnnounceDate	2025-08-11
AnnouncementXML	Submission_2025-08-11_00:40:51.148.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-22 05:27:15	ID requested
⏵ 1	2025-08-11 00:40:51	announced

Dataset with its publication pending

submitter keyword: Chlamydomonas reinhardtii, Synthetic Biology

Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany
contact email	timo.glatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Marburg
contact email	timo.glatter@mpi-marburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD051642

PRIDE project URI

• PRIDE
  1. PXD051642
     1. Label: PRIDE project
     2. Name: High-Throughput Part Characterization and Tool Development to Advance Chloroplast Synthetic Biology