PXD051631-1
PXD051631 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Burkholderia pseudomallei BipD hijacks host KLHL9/KLHL13/CUL3 E3 ligase to ubiquitinate IMMT that initiates mitophagy to evade killing |
| Description | Mitophagy is critical for mitochondrial quality control and function to clear damaged mitochondria. Here, we found that Burkholderia pseudomallei maneuvered host mitophagy for its intracellular survival through the type Ⅲ secretion system needle tip protein BipD. We identified BipD, interacting with BTB-containing proteins KLHL9 and KLHL13 by binding to the Back and Kelch domains, recruited NEDD8 family RING E3 ligase CUL3 in response to B. pseudomallei infection. Although evidently not involved in regulation of infectious diseases, KLHL9/KLHL13/CUL3 E3 ligase complex was essential for BipD-dependent ubiquitination of mitochondria in mouse macrophages. Mechanistically, we discovered the inner mitochondrial membrane IMMT via host ubiquitome profiling as a new substrate of KLHL9/KLHL13/CUL3 complex. Notably, K63-linked ubiquitination of IMMT K211 was required for initiating host mitophagy, thereby reducing mitochondrial ROS production. Together, our findings reveal a unique mechanism used by bacterial pathogens that hijacks host mitophagy for their survival |
| HostingRepository | iProX |
| AnnounceDate | 2024-04-21 |
| AnnouncementXML | Submission_2024-04-22_00:04:02.824.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhi Heng |
| SpeciesList | scientific name: Acyrthosiphon pisum; NCBI TaxID: 7029; scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Velos; LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-22 00:02:55 | ID requested | |
| ⏵ 1 | 2024-04-22 00:04:03 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: BIPD, IP-MS ubiquitinome |
Contact List
| Xiaoyun Liu | |
|---|---|
| contact affiliation | Peking University Health Science Center |
| contact email | xiaoyun.liu@bjmu.edu.cn |
| lab head | |
| Zhi Heng | |
| contact affiliation | Peking University Health Science Center |
| contact email | zhihengt@pku.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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