PXD051619-1

PXD051619 is an original dataset announced via ProteomeXchange.

Title	Striatal proteomics offers clues on the inattention in early adult THRSP-overexpressing mice: Involvement of Snap25 gene
Description	Attention-deficit/hyperactivity disorder, or ADHD, is a neurodevelopmental disorder characterized by inattention, hyperactivity, and impulsivity. Despite its high prevalence, the underlying molecular mechanisms of ADHD remain poorly understood. Recent studies have proposed that changes in the expression of genes involved in regulating synaptic transmission in the striatum may play a role in the pathogenesis of ADHD. To explore the molecular basis of ADHD, we conducted a study using proteomics to analyze whole striatal tissues from early adult thyroid hormone-responsive protein overexpressing (THRSP-OE) mice, which displayed defining characteristics of predominantly inattentive ADHD (ADHD-PI). We focused on the striatal brain region for its critical role in the regulation of attention, motivation, and reward processing. Moreover, the striatum modulates dopaminergic pathways that are known to be impaired in ADHD. Our analysis revealed an innate overexpression of Snap25 protein in THRSP-OE mice, which indicates possible altered SNARE protein complex and potential neurotransmitter dysregulation. Furthermore, a binding affinity study showed reduced dopamine D1 receptor binding concentrations in THRSP-OE mice and pronounced low dopamine levels using enzyme-linked immunosorbent assays measuring DA concentrations. Interestingly, repeated seven-day injections of Methylphenidate improved the low DA, reducing the theta/beta ratio in this animal model. These findings suggest new markers specific to the ADHD-PI presentation and further support the role of Snap25 dysregulation and possible SNARE protein complex alterations in ADHD-PI.
HostingRepository	PRIDE
AnnounceDate	2025-09-22
AnnouncementXML	Submission_2025-09-21_16:57:49.783.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eugene C Yi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-21 19:09:11	ID requested
⏵ 1	2025-09-21 16:57:50	announced

10.1007/s12035-025-05031-z;

Custodio RJP, Sayson LV, Cho A, Jung H, Ortiz DM, Lee HJ, Alyan E, Wascher E, Getzmann S, Kim M, Kim KM, Yi EC, Kim HJ, Cheong JH, Unraveling Predominantly Inattentive ADHD (ADHD-PI): Insights from Proteomic Analysis of the Striatum of Thyroid Hormone-Responsive Protein (THRSP)-Overexpressing Mice. Mol Neurobiol, 62(10):13225-13249(2025) [pubmed]

submitter keyword: Animal model, THRSP-OE mice, SNARE complex, Snap25, Thyroid hormone-response protein,Adult ADHD

Eugene C. Yi
contact affiliation	Seoul National University
contact email	euyi@snu.ac.kr
lab head
Eugene C Yi
contact affiliation	Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University, Republic of Korea
contact email	euyi@snu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051619
     1. Label: PRIDE project
     2. Name: Striatal proteomics offers clues on the inattention in early adult THRSP-overexpressing mice: Involvement of Snap25 gene