PXD051615-1

PXD051615 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of fresh, equilibrated, and frozen/thawed turkey semen in relation to the extracellular fluid proteome
Description	The cryopreservation of avian semen without significant loss of fertilizing ability is of practical interest to the poultry industry and for the conservation of genetic biodiversity. However, the unique physiological features make avian spermatozoa highly sensitive to damage caused by cryopreservation. The proteomic changes in turkey (Meleagris gallopavo) semen throughout the cryopreservation process have never been investigated previously. Therefore, the purpose of the present study was to evaluate the proteomic changes of extracellular fluid (ECF)of semen during cryopreservation at three stages (fresh, equilibrated, and frozen/thawed semen) using 2-dimensional difference in-gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF/TOF). Six protein spots were significantly enriched in ECF proteome after equilibration compared to fresh semen. Twenty seven protein spots were enriched in ECF after cryopreservation compared to fresh semen. Twenty six protein spots were significantly enriched in ECF proteome after cryopreservation compared to equilibrated semen. The proteins enriched in ECF after cryopreservation were mainly related to NADH metabolic and glycolytic process.
HostingRepository	PRIDE
AnnounceDate	2025-07-08
AnnouncementXML	Submission_2025-07-08_04:57:59.649.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariola Słowińska
SpeciesList	scientific name: Meleagris gallopavo (Common turkey); NCBI TaxID: 9103;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-21 11:32:32	ID requested
⏵ 1	2025-07-08 04:58:00	announced

10.1016/j.theriogenology.2025.117473;

Pardyak L, Liszewska E, Judycka S, Machci, ń, ska-Zieli, ń, ska S, Karol H, Dietrich MA, Goj, ł, o E, Arent Z, Bili, ń, ska B, Rusco G, Iaffaldano N, Ciereszko A, S, ł, owi, ń, ska M, The effect of cryopreservation on the turkey (Meleagris gallopavo) semen proteome. Theriogenology, 243():117473(2025) [pubmed]

submitter keyword: turkey, cryopreservation, semen

Mariola Słowińska
contact affiliation	Institute of Animal Reproduction and Food Research of Polish Academy of Sciences
contact email	m.slowinska@pan.olsztyn.pl
lab head
Mariola Słowińska
contact affiliation	Institute of Animal Reproduction and Food Research of Polish Academy of Sciences
contact email	m.slowinska@pan.olsztyn.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD051615

PRIDE project URI

• PRIDE
  1. PXD051615
     1. Label: PRIDE project
     2. Name: Proteomic analysis of fresh, equilibrated, and frozen/thawed turkey semen in relation to the extracellular fluid proteome