PXD051609-1

PXD051609 is an original dataset announced via ProteomeXchange.

Title	Thermal proteome profilling of Ergothionine treated-cells reveals its binding to cystathionine-gamma-lyase (CSE)
Description	Ergothioneine (ET), a dietary thione/thiol, is receiving growing attention for its possible benefits in healthy aging and metabolic resilience. Our study investigates ET's effects on healthspan in aged animals, revealing extension of lifespan and enhanced mobility in Caenorhabditis elegans, accompanied by improved stress resistance and reduced age-associated biomarkers. In aged rats, ET administration enhances exercise endurance, muscle mass, and vascularization, concomitant with higher NAD+ levels in muscle. Mechanistically, ET acts as an alternative substrate for cystathionine gamma lyase (CSE), stimulating H2S production, which increases protein persulfidation of more than 300 protein targets. Among these, protein persulfidation-driven activation of cytosolic glycerol-3-phosphate dehydrogenase (cGPDH) primarily contributes to the ET-induced NAD+ increase. ET’s effects are abolished in models lacking CSE and cGPDH, highlighting essential role of H2S signaling and protein persulfidation. These findings elucidate ET's multifaceted actions and provide insights into its therapeutic potential for combating age-related muscle decline and metabolic perturbations. To better understand the role of ET the cells, we used thermal proteome profilling of cells treated or not with ergothionine, allowing us to find new targets for the compound including CSE.
HostingRepository	PRIDE
AnnounceDate	2025-01-21
AnnouncementXML	Submission_2025-01-20_17:19:17.120.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Vignane
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-20 14:27:09	ID requested
⏵ 1	2025-01-20 17:19:17	announced

10.1016/J.CMET.2024.12.008;

submitter keyword: ergothioneine, healthspan, persulfidation, hydrogen sulfide, NAD

Dr. Milos
contact affiliation	SULFAGING group; Leibniz Institute for Analytica Sciences - ISAS - eV. Dortmund, GERMANY
contact email	milos.filipovic@isas.de
lab head
Thibaut Vignane
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS,e.V, Dortmund, Germany
contact email	thibaut.vignane@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051609
     1. Label: PRIDE project
     2. Name: Thermal proteome profilling of Ergothionine treated-cells reveals its binding to cystathionine-gamma-lyase (CSE)