PXD051602-1

PXD051602 is an original dataset announced via ProteomeXchange.

Title	Global interactomics analysis of the human Duffy antigen receptor for chemokines
Description	The Duffy antigen receptor for chemokines or DARC is a seven trans-membrane glycoprotein that is expressed primarily on the surface of red blood cells. Additionally, it is also expressed on the surface of post-venular endothelial cells, Purkinje cells of the cerebellum, inner ear hair cells, cells of the respiratory pathway and kidney epithelium. DARC binds to more than twenty different chemokines – belonging to both C-C and C-X-C subfamilies – making it the most promiscuous chemokine receptor. However, despite binding to such a large number of chemokines, no G-protein or β-arrestin signaling has been reported downstream to this receptor thus far. While canonical downstream signaling is not likely in erythrocytes considering their anucleate nature, DARC is also expressed in endothelial cells, and therefore, it is plausible that it signals through non-canonical signaling pathways. Considering that DARC does not couple to the canonical GPCR partners, we carried out a global interactome analysis upon stimulation of DARC-expressing HEK-293 cells with CCL7. We first performed a co-immunoprecipitation step under cross-linking condition to enrich DARC-interacting proteins followed by their detection using mass-spectrometry. In these experiments, we also used unstimulated cells as a reference, which were lysed and immunoprecipitated in parallel to subtract non-specific proteins. We carried out these experiments as four independent biological replicates.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:16:42.230.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	gagan jhingan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-20 04:55:37	ID requested
⏵ 1	2024-10-17 05:16:43	announced

10.1016/j.cell.2024.07.005;

Saha S, Khanppnavar B, Maharana J, Kim H, Carino CMC, Daly C, Houston S, Sharma S, Zaidi N, Dalal A, Mishra S, Ganguly M, Tiwari D, Kumari P, Jhingan GD, Yadav PN, Plouffe B, Inoue A, Chung KY, Banerjee R, Korkhov VM, Shukla AK, Molecular mechanism of distinct chemokine engagement and functional divergence of the human Duffy antigen receptor. Cell, 187(17):4751-4769.e25(2024) [pubmed]

submitter keyword: Duffy antigen receptor, G protein-coupled receptors, chemokine receptors, cellular signaling, cryogenic-electron microscopy

Arun Shukla
contact affiliation	Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India
contact email	arshukla@iitk.ac.in
lab head
gagan jhingan
contact affiliation	VProteomics
contact email	gaganjhingan@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051602
     1. Label: PRIDE project
     2. Name: Global interactomics analysis of the human Duffy antigen receptor for chemokines