PXD051592-1

PXD051592 is an original dataset announced via ProteomeXchange.

Title	MYO18B promotes lysosomal exocytosis by facilitating focal adhesion maturation
Description	Many cancer cells exhibit increased amounts of paucimannose glycans, which are truncated N-glycan structures rarely found in mammals. Paucimannosidic proteins are proposedly generated within lysosomes and exposed on the cell surface through a yet uncertain mechanism. In this study, we assured that paucimannosidic proteins in cancer cells are produced by lysosomal glycoside hydrolases and secreted via regulated lysosomal exocytosis, a process in which lysosomes are directly fused with the plasma membrane. Interestingly, lysosomal exocytosis preferentially occurred in the vicinity of focal adhesions, protein complexes connecting the actin cytoskeleton to the extracellular matrix. Through genome-wide knockout screening, we identified that MYO18B, an actin crosslinker, is required for focal adhesion maturation, facilitating lysosomal exocytosis and release of lysosomal proteins to the extracellular milieu. Our data suggest that the mechanosensitive cation channel PIEZO1 locally activated at focal adhesions imports Ca2+ necessary for lysosome-plasma membrane fusion. Collectively, our study unveiled an intimate relationship between lysosomal exocytosis and focal adhesion, shedding light on the unexpected interplay between lysosomal activities and cellular mechanosensing in cancer.
HostingRepository	PRIDE
AnnounceDate	2025-01-14
AnnouncementXML	Submission_2025-01-13_17:22:38.243.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rebeca Kawahara
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-linked glycan core N4-glycosylated asparagine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-19 15:48:06	ID requested
⏵ 1	2025-01-13 17:22:39	announced

Ren WW, Kawahara R, Suzuki KGN, Dipta P, Yang G, Thaysen-Andersen M, Fujita M, MYO18B promotes lysosomal exocytosis by facilitating focal adhesion maturation. J Cell Biol, 224(3):(2025) [pubmed]

10.1083/jcb.202407068;

submitter keyword: paucimannose,Glycoproteomics, lysosome, Hela, HEK293, THP1, MYO18B

Morihisa Fujita
contact affiliation	Institute for Glyco-core Research (iGCORE), Gifu University, Gifu 501-1193, Japan
contact email	fujita.morihisa.h3@f.gifu-u.ac.jp
lab head
Rebeca Kawahara
contact affiliation	Department of Molecular Sciences, Macquarie University, Sydney, NSW, Australia
contact email	rebecasakuma@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD051592

PRIDE project URI

• PRIDE
  1. PXD051592
     1. Label: PRIDE project
     2. Name: MYO18B promotes lysosomal exocytosis by facilitating focal adhesion maturation