PXD051589 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Iron regulatory protein 2 (IRP2) deficiency is protective against resistive breathing-induced pulmonary inflammation |
| Description | Iron regulatory protein 2 (IRP2), a post-transcriptional regulator of cellular iron metabolism has been associated with susceptibility to chronic obstructive pulmonary disease (COPD). To characterize the role of IRP2 in COPD pathogenesis, wild type and Irp2-/- mice were subjected to resistive breathing (RB) via tracheal banding. This experimental model recapitulates the mechanical stress and inflammation in the lungs observed during COPD exacerbations. RB promoted an increase in tissue elasticity and hysteresis in wild type but not in Irp2-/- mice, denoting that the latter were protected against lung injury. Moreover, Irp2-/- mice exhibited a lower score of lung inflammation. Additionally, only wild type but not Irp2-/- mice responded to RB by increasing bronchoalveolar lavage (BAL) cellularity due to higher macrophage count, which was accompanied by elevated BAL IL-1β and IL-6 levels. Lung proteomics and functional enrichment analysis revealed significant differences among wild type and Irp2-/- mice in RB-induced regulation of proteins involved in inflammatory and defense response pathways, but also of many proteins with unknown function. We conclude that IRP2 supports pro-inflammatory activities, while its deficiency protects against RB-induced pulmonary inflammation via remodeling of the lung proteome. Our data suggest that IRP2 is a bona fide pharmacological target against COPD. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-15 |
| AnnouncementXML | Submission_2025-12-14_19:21:21.475.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Vincent Richard |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-04-19 13:44:48 | ID requested | |
| ⏵ 1 | 2025-12-14 19:21:21 | announced | |
Publication List
Keyword List
| submitter keyword: COPD,iron regulatory protein 2, restrictive breathing, IRP2 |
Contact List
| Christoph Borchers |
|---|
| contact affiliation | Segal Cancer Proteomics Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montréal, Quebec, H3T 1E2, Canada Division of Experimental Medicine, McGill University, Montréal, Quebec H4A 3J1, Canada Gerald Bronfman Department of Oncology, McGill University, Montréal, Quebec H4A 3T2, Canada |
| contact email | christoph.borchers@mcgill.ca |
| lab head | |
| Vincent Richard |
|---|
| contact affiliation | Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University |
| contact email | vincentroyrichard@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051589
- Label: PRIDE project
- Name: Iron regulatory protein 2 (IRP2) deficiency is protective against resistive breathing-induced pulmonary inflammation
