PXD051587-1

PXD051587 is an original dataset announced via ProteomeXchange.

Title	Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation
Description	Ergothioneine (ET), a dietary thione/thiol, is receiving growing attention for its possible benefits in healthy aging and metabolic resilience. Our study investigates ET's effects on healthspan in aged animals, revealing extension of lifespan and enhanced mobility in Caenorhabditis elegans, accompanied by improved stress resistance and reduced age-associated biomarkers. In aged rats, ET administration enhances exercise endurance, muscle mass, and vascularization, concomitant with higher NAD+ levels in muscle. Mechanistically, ET acts as an alternative substrate for cystathionine gamma lyase (CSE), stimulating H2S production, which increases protein persulfidation of more than 300 protein targets. Among these, protein persulfidation-driven activation of cytosolic glycerol-3-phosphate dehydrogenase (cGPDH) primarily contributes to the ET-induced NAD+ increase. ET’s effects are abolished in models lacking CSE and cGPDH, highlighting essential role of H2S signaling and protein persulfidation. These findings elucidate ET's multifaceted actions and provide insights into its therapeutic potential for combating age-related muscle decline and metabolic perturbations.
HostingRepository	PRIDE
AnnounceDate	2025-01-21
AnnouncementXML	Submission_2025-01-21_15:30:20.347.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051587
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dunja Petrovic
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-19 12:25:13	ID requested
⏵ 1	2025-01-21 15:30:20	announced

10.6019/PXD051587;

10.1016/J.CMET.2024.12.008;

submitter keyword: ergothioneine, healthspan, persulfidation, hydrogen sulfide, NAD

Milos Filipovic
contact affiliation	Sulfaging group, Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V., Dortmund, Germany
contact email	milos.filipovic@isas.de
lab head
Dunja Petrovic
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact email	dunja.petrovic@isas.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051587
     1. Label: PRIDE project
     2. Name: Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation