PXD051583-1

PXD051583 is an original dataset announced via ProteomeXchange.

Title	Signal peptide-independent secretion of keratin-19 by pancreatic cancer cells
Description	T cells exclusion precludes checkpoint inhibition efficacy in pancreatic ductal adenocarcinoma (PDA) and is mediated by the interaction between T cell CXCR4, and its ligand CXCL12, which is complexed to keratin-19 (KRT19) on the surface of PDA cells. KRT19 secretion is essential to this process but is unusual because KRT19 lacks an endoplasmic reticulum (ER)-directing signal peptide (SP). By using ER-restricted TurboID sys-tems and a split-GFP assay, we demonstrate that KRT19 enters the ER via its “head” domain. In vivo, tumors formed with ER-TurboID cells contain biotinylated KRT19. Additionally, KRT19 is shown to interact with the sig-nal recognition particle and its secretion is sensitive to canonical protein secretion inhibitors. Although keratin-8 (KRT8) co-localizes with KRT19 on the surface of PDA cells, KRT8 does not enter the ER. However, both keratins are externalized via secretory autophagy. Thus, despite lacking a classical SP, PDA cells secrete KRT19 to protect against immune attack.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-16_06:30:46.804.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paolo Cifani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-19 10:23:52	ID requested
⏵ 1	2025-06-16 06:30:47	announced

Dataset with its publication pending

submitter keyword: Keratin19

pancreatic ductal adenocarcinoma

immunoprecipitation

ingel

Paolo Cifani
contact affiliation	Cold Spring Harbor Laboratory
contact email	cshl.mass.spec@gmail.com
lab head
Paolo Cifani
contact affiliation	Cold Spring Harbor Laboratory
contact email	cshl.mass.spec@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051583
     1. Label: PRIDE project
     2. Name: Signal peptide-independent secretion of keratin-19 by pancreatic cancer cells