PXD051567-1
PXD051567 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cold Storage Disrupts the Proteome Landscape in Rat Kidney Transplants |
| Description | Approximately 70% of kidney grafts are obtained from deceased donors, and these grafts must be preserved in hypothermic conditions to prolong their viability until transplantation. However, prolonged cold storage (CS) of kidneys results in poor long-term outcomes after transplantation. We reported previously that CS of rat kidneys for 18 h prior to transplant impaired proteasome function, disrupted protein homeostasis, and reduced graft function. The goal of the present study was to identify the renal proteins that are dysregulated by this CS-induced injury. Isolated donor Lewis rat kidneys were subject to 18-h CS and transplanted into recipient Lewis rats (CS+Tx). Autotransplantation (ATx: transplant with 0-h CS) or Sham (right nephrectomy) surgeries served as controls. The proteome of kidney homogenates was analyzed with tandem mass-tag mass spectrometry to identify CS-induced abnormalities in kidney grafts. CS injury disrupted the renal phosphoproteome in kidney grafts and dysregulated numerous signaling pathways. Integrated analysis of global proteomes and phosphoproteomes identified 15 proteins that were significantly regulated in a CS-specific manner. In particular, proteins and pathways such as complement and coagulation cascades were upregulated, while antioxidant pathways, such as glutathione, were suppressed in CS+Tx groups compared to ATx and Sham controls. This study, for the first time, provides deeper insight into the disruption of the renal graft proteome caused by CS injury and provides a novel set of pathways and molecules that can be investigated to delineate their specific role in renal transplant outcomes, ultimately improving outcomes for patients with end-stage kidney disease. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-04-18 |
| AnnouncementXML | Submission_2024-04-18_13:21:36.212.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Stephanie Byrum |
| SpeciesList | scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116; |
| ModificationList | Oxidation |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-18 10:52:52 | ID requested | |
| ⏵ 1 | 2024-04-18 13:21:36 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Kidney transplant, cold storage |
Contact List
| Nirmala Parajuli | |
|---|---|
| contact affiliation | University of Arkansas for Medical Sciences |
| contact email | nparajuli@uams.edu |
| lab head | |
| Stephanie Byrum | |
| contact affiliation | University of Arkansas for Medical Sciences |
| contact email | sbyrum@uams.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094566/ |
to receive all new ProteomeXchange dataset release announcements!
