PXD051562-1
PXD051562 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A comparison of acetic and formic acid in single H358 cells analyzed by diaPASEF |
| Description | Single human H358 cancer cells were isolated by flow cytometry into 96 well plates containing 1 uL of LCMS grade acetonitrile. The cells were placed immediately on dry ice and transported to the lab where they were stored at minus 80C. Dried cell lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1percent n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB. Two microliters of trypsin solution were used for each cell prior to the plate being tightly sealed with adhesive plate tape (Fisher, 60180-M143) and room temperature overnight digestion. Following digestion, the peptide digest was briefly centrifuged to condense evaporation and the plates were completely dried with vacuum centrifugation. The peptides were resuspended in 3.5 uL of 0.1 percent formic acid, vortexed and centrifuged prior to loading on the autosampler. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-04-18 |
| AnnouncementXML | Submission_2024-04-18_11:42:12.186.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | ben |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation |
| Instrument | timsTOF SCP |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-18 09:00:44 | ID requested | |
| ⏵ 1 | 2024-04-18 11:42:12 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Single cell proteomics, diaPASEF, Buffer modifiers |
Contact List
| Benjamin C Orsburn | |
|---|---|
| contact affiliation | Johns Hopkins |
| contact email | borsbur1@jhmi.edu |
| lab head | |
| ben | |
| contact affiliation | The Johns Hopkins School of Medicine |
| contact email | ben_orsburn3@hotmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094565/ |
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