PXD051561-1
PXD051561 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | High-density CRISPRi screens reveal adaptive transcriptional gradients in cyanobacteria |
| Description | Cyanobacteria are the oldest form of photosynthetic life and contribute to primary production in nearly every habitat, from permafrost to hot springs. Despite longstanding interest in the biochemical basis of environmental adaptation in these microbes, it remains poorly understood. Here we used a high-density, genome-wide CRISPRi screen to examine the influence of gene-specific transcriptional variation on the growth of Synechococcus sp. PCC 7002 under environmental extrema. Surprisingly, many partial knockdowns enhanced fitness under cold monochromatic conditions. Notably, transcriptional repression of a gene for a core subunit of the NDH-1 complex, which is important for photosynthesis and carbon uptake, improved growth rates under both red and blue light but at distinct, color-specific optima. Multi-target transcriptional repression produced nonadditive effects. Findings reveal diverse mechanisms of environmental adaptation in cyanobacteria and provide a new approach for using gradients in sgRNA activity to pinpoint biochemically influential transcriptional changes in cells. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-08-29 |
| AnnouncementXML | Submission_2024-08-29_14:03:23.944.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dana Carper |
| SpeciesList | scientific name: Synechococcus sp. PCC 7002; NCBI TaxID: 32049; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-18 08:01:15 | ID requested | |
| ⏵ 1 | 2024-08-29 14:03:24 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Cyanobacteria, Proteomics |
Contact List
| Paul Abraham | |
|---|---|
| contact affiliation | ORNL |
| contact email | pav@ornl.gov |
| lab head | |
| Dana Carper | |
| contact affiliation | Oak Ridge National Laboratory |
| contact email | carperdl@ornl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094564/ |
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