PXD051521-1

PXD051521 is an original dataset announced via ProteomeXchange.

Title	Deep phosphoproteomics of Klebsiella pneumoniae reveals HipA-mediated tolerance to ciprofloxacin
Description	Klebsiella pneumoniae belongs to the group of bacterial pathogens causing the majority of antibiotic-resistant nosocomial infections worldwide; however, the molecular mechanisms underlying post-translational regulation of its physiology are poorly understood. Here we perform a comprehensive analysis of Klebsiella phosphoproteome, focusing on HipA, a Ser/Thr kinase involved in antibiotic tolerance in Escherichia coli. We show that overproduced K. pneumoniae HipA (HipAkp) is toxic to both E. coli and K. pneumoniae and its toxicity can be rescued by overproduction of the antitoxin HipBkp. Importantly, HipAkp overproduction leads to increased tolerance against ciprofloxacin, a commonly used antibiotic in the treatment of K. pneumoniae infections. Proteome and phosphoproteome analyses in the absence and presence of ciprofloxacin confirm that HipAkp has Ser/Thr kinase activity, auto-phosphorylates at S150, and shares multiple substrates with HipAec, thereby providing a valuable resource to clarify the molecular basis of tolerance and the role of Ser/Thr phosphorylation in this human pathogen.
HostingRepository	PRIDE
AnnounceDate	2024-11-21
AnnouncementXML	Submission_2024-11-21_03:36:24.785.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Payal Nashier
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Klebsiella pneumoniae subsp. pneumoniae; NCBI TaxID: 72407;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-17 00:20:43	ID requested
⏵ 1	2024-11-21 03:36:25	announced

10.1371/JOURNAL.PPAT.1012759;

submitter keyword: Phosphoproteome

Klebsiella pneumoniae

Antibiotic tolerance

Persistence

Mass Spectrometry

Boris Macek
contact affiliation	Proteome Center Tuebingen, Institute of Cell Biology, University of Tuebingen, Tuebingen, Germany
contact email	boris.macek@uni-tuebingen.de
lab head
Payal Nashier
contact affiliation	Proteome Center Tuebingen, Institute of Cell Biology, University of Tuebingen
contact email	payal.nashier@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051521
     1. Label: PRIDE project
     2. Name: Deep phosphoproteomics of Klebsiella pneumoniae reveals HipA-mediated tolerance to ciprofloxacin