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PXD051518-1

PXD051518 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnnotation of the genes responsible for helix 69 rRNA modifications in 23S of Bacillus subtilis.
DescriptionA total of 25 modifications were mapped to 16S and 23S RNA in B. subtilis ribosome, however most of the genes responsible for these modification sites remain unknown. In this work, bottom-up oligonucleotide LC-MS/MS was used to detect rRNA modifications in five single gene knock-out strains: yydA, yhcT, yjbO, ylyB, yqxC. By comparing oligonucleotide modification states between the background 168 strain (annotated as WT) and the mutants: ylyB was confirmed to be a pseudouridine synthase at positions 1940, 1944, 1946 of 23S; yydA is a N3-pseudouridine methyltransferase at 1944 of 23S; and yqxC is a dual 2’O-cytidine methyltransferase at 1417 of 16S and 1949 of 23S. Deletion of yhcT or yjbO does not change rRNA modification pattern.
HostingRepositoryPRIDE
AnnounceDate2024-08-06
AnnouncementXMLSubmission_2024-08-06_01:08:05.681.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnna Popova
SpeciesList scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationListmonomethylated residue
Instrumentimpact II
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-16 23:35:48ID requested
12024-08-06 01:08:06announced
22024-10-22 06:52:32announced2024-10-22: Updated project metadata.
Publication List
10.1093/NAR/GKAE626;
Keyword List
submitter keyword: pseudouridine, base and ribose methylations,23S, RNA modifications, Bacillus subtilis., ribosomal RNA
Contact List
James Williamson
contact affiliationProfessor, Department of Integrative Structural & Computational Biology, Department of Chemistry at the Scripps Research Institute
contact emailjrwill@scripps.edu
lab head
Anna Popova
contact affiliationScripps Research
contact emailpopova@scripps.edu
dataset submitter
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Dataset FTP location
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