PXD051516 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Functional antagonism between Mir483 and its host gene Igf2 in the control of developmental growth |
Description | Many mammalian microRNAs are embedded within introns of protein-coding genes, yet their functional relationship with the host gene is poorly understood. Here we identify Mir483, as having opposing developmental and physiological roles to those of its host gene, the paternally-expressed Igf2. Mechanistically, Mir483 expression is dependent on Igf2 transcription and the epigenetic regulation of the Igf2/H19 imprinting control region. Mir483 deletion in vivo resulted in normal growth, but induced distinct molecular signatures. Transgenic Mir483 overexpression in utero caused fetal, but not placental, growth restriction and cardiovascular defects leading to fetal demise. Overexpression of Mir483 postnatally resulted in growth stunting through IGF1 repression, increased hepatic lipid production, and excessive adiposity. IGF1 infusion rescued the post-natal growth restriction. Our data highlight a novel functional antagonism between a growth-suppressor microRNA and its growth-promoting host gene. We suggest that Mir483 was evolutionary co-opted to provide exquisite control of IGF signalling activity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:58:11.742.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Robin Antrobus |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-16 20:56:33 | ID requested | |
1 | 2024-09-12 10:13:49 | announced | |
⏵ 2 | 2024-10-22 06:58:12 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: growth,Mouse, TMT, microRNA, quantitative proteomics |
Contact List
Miguel Constância |
contact affiliation | 1Medical Research Council Metabolic Diseases Unit, Institute of Metabolic Science-Metabolic Research Laboratories, University of Cambridge, Cambridge, United Kingdom |
contact email | jmasmc2@cam.ac.uk |
lab head | |
Robin Antrobus |
contact affiliation | CIMR medicine |
contact email | pra29@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051516
- Label: PRIDE project
- Name: Functional antagonism between Mir483 and its host gene Igf2 in the control of developmental growth