PXD051495 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cytokinetic Abscission in Toxoplasma gondii is governed by Protein Phosphatase 2A and the Daughter Cell Scaffold Complex |
Description | Cytokinetic abscission is the last step of cell division during which the daughter cells physically separate through the generation of barriers in the form of new plasma membrane or cell wall. While the contractile ring is a prominent structure during cytokinesis in bacteria, fungi and animal cells, the mechanism is divergent in Apicomplexa. In Toxoplasma gondii, two daughter cells are formed within the intact mother cell by endodyogeny. The acquisition of plasma membrane at the end of the division cycle occurs by an unknown mechanism, when the mother cell disassembles, and the daughter cells emerge. Here we identified and functionally characterized the essential subunits of a predicted PP2A complex in T. gondii, named PP2A-2. The three subunits PP2A-A2, -B2 and -C2 exhibit a dynamic localization during parasite division. Their individual downregulation prevents the accumulation of plasma membrane at the division plane, resulting in a block of cytokinesis. Remarkably, the absence of cytokinetic abscission does not preclude division cycles and the consecutive progeny is able to successfully egress from the infected cells but fails to glide andinvade new host cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-13 |
AnnouncementXML | Submission_2024-06-13_07:54:25.767.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gaelle Lentini |
SpeciesList | scientific name: Toxoplasma gondii; NCBI TaxID: 5811; scientific name: Toxoplasma gondii; NCBI TaxID: NCBITaxon:5811; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-16 09:45:20 | ID requested | |
⏵ 1 | 2024-06-13 07:54:26 | announced | |
2 | 2024-08-27 02:43:45 | announced | 2024-08-27: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Toxoplasma gondii, cellular abscission, tachyzoites, PP2A complex, LC-ESI- MS/MS |
Contact List
Gaƫlle Lentini |
contact affiliation | Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland |
contact email | gaelle.lentini@unige.ch |
lab head | |
Gaelle Lentini |
contact affiliation | Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland. Institute of Cell Biology, University of Bern, Bern, Switzerland |
contact email | gaelle.lentini@unige.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051495
- Label: PRIDE project
- Name: Cytokinetic Abscission in Toxoplasma gondii is governed by Protein Phosphatase 2A and the Daughter Cell Scaffold Complex