PXD051492-1

PXD051492 is an original dataset announced via ProteomeXchange.

Title	Glycosylation profiling of Influenza A Virus H3 HA
Description	The quest for a universal influenza vaccine holds great promise for mitigating the global burden of influenza-related morbidity and mortality. Despite significant progress, challenges persist in identifying conserved epitopes capable of inducing broadly protective immune responses, understanding the correlates of protection against influenza infection, and ensuring vaccine safety and efficacy across diverse populations. In this study, we explore the influence of glycan evolution on H3 HA from 1968 to the present. Our in-depth analysis unveiled how glycan addition significantly and differentially impacted HA antigenicity and immunogenicity over time. We observe that adding glycans influenced the polyclonal immune response elicited post-immunization. We propose that the appearance of PNGS at N158 potentially impacted the binding of head-specific broadly neutralizing antibodies (bnAbs). We further observe that, while enzymatic de-glycosylation barely impacted HK/68 HA stability, it destabilized and unfolded in the hyperglycosylated head domain and the membrane-proximal region of the stem domain in Sing/16. These insights expand our understanding of glycans beyond their conventional role in protein folding and as immune evasion tactics and highlight the intricate interplay among glycan integration, viral fitness, and immune recognition. Our findings have implications for vaccine design and manufacturing processes, offering valuable insights for developing more effective influenza vaccines. Moreover, they contribute to ongoing efforts to refine influenza research strategies, emphasizing the potential of stem-directed antibodies in creating more effective and universally applicable influenza vaccines.
HostingRepository	PRIDE
AnnounceDate	2026-01-12
AnnouncementXML	Submission_2026-01-11_16:52:57.743.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joost Snijder
SpeciesList	scientific name: Influenza A virus; NCBI TaxID: NEWT:11320;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-16 07:33:03	ID requested
⏵ 1	2026-01-11 16:52:58	announced

10.1038/s41467-025-66375-7;

de Paiva Froes Rocha R, Tomris I, Bowman CA, Stevens E, Kantorow J, Plitt CM, Peng W, Oeverdieck S, Galdino Andrade T, Ferguson JA, Jung DD, Marques RE, Herfst S, Snijder J, Chakraborty S, Torrents de la Pe, ñ, a A, Berndsen ZT, de Vries RP, Ward AB, Structural and immunological characterization of the H3 influenza hemagglutinin during antigenic drift. Nat Commun, 16(1):11452(2025) [pubmed]

submitter keyword: glycosylation

influenza;

Joost Snijder
contact affiliation	Utrecht University
contact email	j.snijder@uu.nl
lab head
Joost Snijder
contact affiliation	Utrecht University
contact email	joost.snijder@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD051492

PRIDE project URI

• PRIDE
  1. PXD051492
     1. Label: PRIDE project
     2. Name: Glycosylation profiling of Influenza A Virus H3 HA