PXD051489-1

PXD051489 is an original dataset announced via ProteomeXchange.

Title	Spatiotemporal proteomic profiling of cellular responses to NLRP3 agonists
Description	Here, we mapped subcellular proteome changes to lysosomes, mitochondrion, EEA1-positive endosomes, and Golgi caused by the NLRP3 inflammasome agonists nigericin and CL097. We identified a number of common disruptions to retrograde trafficking pathways, including COPI and Shiga toxin-related transport, in line with recent studies. We further characterized mouse NLRP3 trafficking throughout its activation using temporal proximity proteomics, which supports a recent model of NLRP3 recruitment to endosomes during inflammasome activation. Collectively, these findings provide additional granularity to our understanding of the molecular events driving NLRP3 activation and serve as a valuable resource for cell biological research.
HostingRepository	PRIDE
AnnounceDate	2024-04-23
AnnouncementXML	Submission_2024-04-23_08:33:43.886.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louis Hollingsworth
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-16 06:02:08	ID requested
⏵ 1	2024-04-23 08:33:44	announced
2	2024-10-22 06:37:13	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: inflammasome, organellar damage,NLRP3, spatial proteomics

J. Wade Harper
contact affiliation	Department of Cell Biology, Harvard Medical School
contact email	wade_harper@hms.harvard.edu
lab head
Louis Hollingsworth
contact affiliation	Department of Cell Biology, Harvard Medica School
contact email	bobbyh11@vt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD051489

PRIDE project URI

• PRIDE
  1. PXD051489
     1. Label: PRIDE project
     2. Name: Spatiotemporal proteomic profiling of cellular responses to NLRP3 agonists