PXD051479-1

PXD051479 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of ROCK Inhibitor-treated ARPE-19 Cells
Description	Rho-associated protein kinase (ROCK) inhibitors are highly present in different ocular tissues, and upregulation of ROCK pathway has been shown related to the pathogenesis of multiple ocular disorders, including glaucoma, diabetic retinopathy and age-related macular degeneration (AMD). However, the effect of ROCK inhibitor on AMD is still unknown. The protein profile changes in human retinal pigment epithelium (ARPE-19) cells after two days treatment with a ROCK inhibitor were studied using label-free Zeno SWATH acquisition. These results provided significant data of key protein candidates underlying the effect of ROCK inhibitor. Using the sensitive label-free mass spectrometry approach with data-independent acquisition (SWATH-MS), we established a comprehensive ARPE-19 proteome library. This project describes the use of Zeno SWATH MS to examine the underlying biological protein changes following ROCK inhibitor treatment of APRE-19 cells. This knowledge may allow more specific anti-AMD drugs to be developed
HostingRepository	PRIDE
AnnounceDate	2024-12-04
AnnouncementXML	Submission_2024-12-03_18:03:39.489.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kirk Patrick Catral
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-16 02:02:35	ID requested
⏵ 1	2024-12-03 18:03:39	announced

10.3390/CELLS13221907;

submitter keyword: Autophagy,RPE, OneOmics, ROCK inhibitor, Zeno SWATH

Samantha Sze-Wan Shan
contact affiliation	School of Optometry, the Hong Kong Polytechnic University, Hong Kong, China Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong Kong, China Research Centre for SHARP Vision (RCSV), the Hong Kong Polytechnic University, Hong Kong, China Research Centre for Chinese Medicine (RCMI), the Hong Kong Polytechnic University, Hong Kong, China
contact email	samantha.shan@polyu.edu.hk
lab head
Kirk Patrick Catral
contact affiliation	The Hong Kong Polytechnic University
contact email	kpatrick.catral@connect.polyu.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD051479

PRIDE project URI

• PRIDE
  1. PXD051479
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of ROCK Inhibitor-treated ARPE-19 Cells