PXD051462-1

PXD051462 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis reveals insights into the role of lipid metabolism in T2DM-related sarcopenia
Description	Sarcopenia has been recognized as an emerging complication of type 2 diabetes mellitus (T2DM). Currently, the pathogenesis of T2DM-related sarcopenia remains unclear. The aim of this study was to investigate the molecular mechanisms and potential therapeutic targets for T2DM-related sarcopenia. In this study, a T2DM-related sarcopenia mouse model was established using db/db mice. Proteins extracted from the gastrocnemius muscles of db/db mice and littermate control db/m mice were analyzed by a 4D label-free quantitative proteomics approach. A total of 131 upregulated and 68 downregulated proteins were identified as differentially expressed proteins (DEPs). Bioinformatics analysis revealed that DEPs were significantly enriched in lipid metabolism. Protein–protein interaction network analysis revealed that six hub proteins, including ACOX1, CPT2, ECI2, ACADVL, ACADL, and ECH1, were involved in the fatty acid oxidation. The hub protein-transcription factor-miRNA network was also constructed through the NetworkAnalyst tool. Finally, the hub proteins were validated by western blotting and immunohistochemistry and further confirmed to be significantly negatively correlated with the muscle mass and grip strength. Our study suggested that lipid metabolism, especially excessive fatty acid oxidation, may be a crucial contributor to the progression of T2DM-related sarcopenia and a common cause of the interrelationship between T2DM and sarcopenia.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:18:17.175.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wu Jingying
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-15 19:52:54	ID requested
⏵ 1	2024-10-17 05:18:18	announced

10.1021/acsomega.4c04668;

Wu J, Wang S, Zhuang H, Wang W, Wang Y, Chen Y, Huang Z, Chen C, Chen X, Proteomics Analysis Provides Insights into the Role of Lipid Metabolism in T2DM-Related Sarcopenia. ACS Omega, 9(31):34056-34069(2024) [pubmed]

submitter keyword: proteomics, lipid metabolism, T2DM-related sarcopenia, fatty acid oxidation, diabetes mellitus,sarcopenia

Jingying Wu
contact affiliation	Department of Cardiology, The Second Affiliated Hospital of Fujian Medical University, 950 Donghai Street, Fengze District, Quanzhou, Fujian Province, 362000, China.
contact email	wjingying868@163.com
lab head
Wu Jingying
contact affiliation	The Second Affiliated Hospital, Fujian Medical University
contact email	wjingying868@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD051462

PRIDE project URI

• PRIDE
  1. PXD051462
     1. Label: PRIDE project
     2. Name: Proteomics analysis reveals insights into the role of lipid metabolism in T2DM-related sarcopenia