PXD051442-1

PXD051442 is an original dataset announced via ProteomeXchange.

Title	Luminescent sensing of conformational integrin activation in living cells
Description	Integrins are the major receptors for secreted extracellular matrix, playing crucial roles in several physiological and pathological contexts, such as angiogenesis and cancer. Regulation of the transition between inactive and active conformation is key for integrins to fulfill their functions, and pharmacological control of those dynamics may have therapeutic applications. We created and validated a prototypic luminescent β1 integrin activation sensor (β1IAS) by introducing a split luciferase in a functionally critical extracellular site between the βI and the hybrid domains. Both as recombinant protein and in living cells, β1IAS accurately reports β1 integrin activation in response to (bio)chemical and physical stimuli. A siRNA high-throughput screening on live β1IAS knockin endothelial cells unveiled novel actionable regulators of β1 integrin activation, such as the E3 ligase PJA2 and VEGFB. This split luciferase-based strategy may allow generating activation sensors of other β integrins and receptors.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_14:03:37.360.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kelly Hodge
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-15 04:12:19	ID requested
⏵ 1	2025-05-06 14:03:38	announced

10.1016/j.celrep.2025.115319;

Villari G, Gioelli N, Gino M, Zhang H, Hodge K, Cordero F, Zanivan S, Zhu J, Serini G, Luminescent sensing of conformational integrin activation in living cells. Cell Rep, 44(2):115319(2025) [pubmed]

submitter keyword: TMT, human,VEGFB, Proteomics

Sara Zanivan
contact affiliation	Head of Proteomics, CRUK Scotland Institute, Glasgow
contact email	sara.zanivan@glasgow.ac.uk
lab head
Kelly Hodge
contact affiliation	CRUK Beatson Institute
contact email	k.hodge@beatson.gla.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051442
     1. Label: PRIDE project
     2. Name: Luminescent sensing of conformational integrin activation in living cells