PXD051390 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of extracellular vesicles in human seminal plasma: a multiomic analysis |
| Description | Extracellular Vesicles (EVs) derived from seminal plasma have achieved considerable attention due to their potential role in male reproductive physiology and pathology. In this study, we employed a comprehensive proteomic and transcriptomic approach to investigate the molecular signatures of EVs isolated from human seminal plasma. EVs from Normozoospermic (NORMO,15), OligoAsthenoTeratozoospermic (OAT,12) and Azoospermic (AZO,12) subjects, were isolated by an in house modified polymer precipitation-based protocol and characterized for their size and morphology by NTA and TEM. Full proteomic analysis, by gel-free and gel-based approaches, revealed distinct protein profiles in each group, highlighting potential molecules and pathways implicated in sperm function and fertility. Furthermore, transcriptomic analysis confirmed the trend of general down-regulation of AZO and OAT groups compared to NORMO offering insights into the regulatory mechanisms underlying sperm development and function. Bioinformatic tools were applied for functional Omics analysis; integration of proteomic and transcriptomic data provided a comprehensive understanding of the cargo content and regulatory networks. This study contributes to elucidating the key role of EVs in the paracrine communication regulating spermatogenesis. The full understanding of these pathways not only suggests potential mechanisms regulating male fertility but also offers new insight for the development of diagnostic tools targeting male reproductive disorders. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-06-30 |
| AnnouncementXML | Submission_2025-06-29_16:05:45.961.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Enxhi Shaba |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | ultraflex |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-04-12 03:25:12 | ID requested | |
| ⏵ 1 | 2025-06-29 16:05:46 | announced | |
Publication List
| Governini L, Haxhiu A, Shaba E, Vantaggiato L, Mori A, Bruttini M, Loria F, Zarovni N, Piomboni P, Landi C, Luddi A, Unraveling the Multi-Omic Landscape of Extracellular Vesicles in Human Seminal Plasma. Biomolecules, 15(6):(2025) [pubmed] |
| 10.3390/biom15060836; |
Keyword List
| submitter keyword: extracellular vesicles |
| seminal plasma |
| multi-omics |
| proteomics |
| transcriptomics |
Contact List
| Prof. Claudia Landi |
|---|
| contact affiliation | Functional Proteomics Lab, Dpt. Life Sciences, University of Siena, Italy |
| contact email | claudia.landi@unisi.it |
| lab head | |
| Enxhi Shaba |
|---|
| contact affiliation | Functional Proteomics Lab, Life Sciences Department, University of Siena |
| contact email | enxhi.shaba@unisi.it |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051390
- Label: PRIDE project
- Name: Characterization of extracellular vesicles in human seminal plasma: a multiomic analysis
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