PXD051352-1
PXD051352 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | tRNA-m1A Methylation Regulates the Infection of Magnaporthe oryzae by Supporting Ergosterol Biosynthesis |
| Description | Ergosterols are essential components of fungal plasma membranes. Inhibitors targeting ergosterol biosynthesis (ERG) genes are critical for controlling fungal pathogens, including Magnaporthe oryzae, the fungus that causes rice blast. In this study we show that the Trm6/Trm61 complex catalyzes dynamic N1-methyladenosine at position 58 (m1A58) in 51 tRNAs of M. oryzae, significantly influencing translation at both initiation and elongation stages. The absence of m1A58 leads to substantial decreases in the translation of ERG genes, ergosterol production, and consequently, fungal virulence. Strikingly, simultaneously targeting the Trm6/Trm61 complex and the ergosterol biosynthesis pathway markedly improves control against rice blast. Our findings demonstrate the pivotal role of m1A58-mediated translational regulation in ergosterol production and fungal infection, offering a new strategy for fungicide development. Mass spectrometry data used in this paper are included in these uploaded files. |
| HostingRepository | iProX |
| AnnounceDate | 2024-06-07 |
| AnnouncementXML | Submission_2024-12-12_18:30:50.002.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhipeng Zhou |
| SpeciesList | scientific name: Pyricularia oryzae 70-15; NCBI TaxID: 242507; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-11 00:25:10 | ID requested | |
| ⏵ 1 | 2024-12-12 18:30:50 | announced |
Publication List
| He R, Lv Z, Li Y, Ren S, Cao J, Zhu J, Zhang X, Wu H, Wan L, Tang J, Xu S, Chen XL, Zhou Z, A methylation controls the infection of Magnaporthe oryzae by supporting ergosterol biosynthesis. Dev Cell, 59(22):2931-2946.e7(2024) [pubmed] |
Keyword List
| submitter keyword: fungal pathogens, Magnaporthe oryzae, ergosterol biosynthesis |
| translational regulation, tRNA modification, m1A methylation |
Contact List
| Zhipeng Zhou | |
|---|---|
| contact affiliation | Huazhong Agricultural University |
| contact email | zhouzhipeng9@126.com |
| lab head | |
| Zhipeng Zhou | |
| contact affiliation | Huazhong Agricultural University |
| contact email | zhouzhipeng9@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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