PXD051342-1

PXD051342 is an original dataset announced via ProteomeXchange.

Title	Identification of HELLS as a Novel DNA G-quadruplex Helicase
Description	Guanine quadruplexes (G4s) are unique secondary structures of nucleic acids with functions in many biological processes. Understanding the biological functions of DNA G4s requires the knowledge about their recognitions by cellular proteins. Affinity pull-down coupled with LC-MS/MS analysis was previously employed to identify G4-binding proteins (G4BPs); the approach, however, has limitations in capturing weak and transient interactions, potentially leading to false-positives. Here, we developed a photoclick chemistry-based method, in combination with LC-MS/MS, for uncovering G4BPs. By incorporating a photoactivatable ortho-nitrobenzylamine (o-NBA) moiety into G4 DNA probes and employing UVA irradiation along with stringent washing, we identified 99 proteins enriched with G4 structures derived from the human telomere. We further validated the abilities of one of these proteins, HELLS, in binding and resolving G4 structures both in vitro and in chromatin. Together, we developed a photoclick chemistry-based approach for identifying novel G4BPs. Our work led to the discovery of new G4BPs, and uncovered novel functions of HELLS in recognizing and unwinding G4 structures in vitro and in cells.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_16:21:52.947.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zi Gao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-10 16:08:16	ID requested
⏵ 1	2026-02-01 16:21:55	announced

10.1093/nar/gkag034;

Gao Z, Chong Y, He X, Yuan J, Wang Y, Photoclick chemistry led to the identification of HELLS as a helicase for DNA G-quadruplexes. Nucleic Acids Res, 54(3):(2026) [pubmed]

submitter keyword: LC-MS/MS, G-quadruplex, HeLa

Zi Gao
contact affiliation	Department of Chemistry, University of California Riverside, Riverside, CA 92521-0403.
contact email	zgao015@ucr.edu
lab head
Zi Gao
contact affiliation	University of California, Riverside
contact email	zgao015@ucr.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051342
     1. Label: PRIDE project
     2. Name: Identification of HELLS as a Novel DNA G-quadruplex Helicase