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PXD051328-1

PXD051328 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFluorescence imaging sheds light on the immune evasion mechanisms of hepatic stellate cells mediated by superoxide anion
DescriptionWhether and how the reactive oxygen species generated by hepatic stellate cells (HSCs) promote immune evasion of hepatocellular carcinoma (HCC) remains mysteriouss. Therefore, investigating the function of superoxide anion, the firstly generated reactive oxygen species, during the immune evasion become necessary. In this work, we establish a novel in situ imaging method for visualization of superoxide anion changes in HSCs based on a new two-photon fluorescence probe TPH. TPH comprises recognition group for superoxide anion and HSCs targeting peptides. We observe that superoxide anion in HSCs gradually rose, impairing the infiltration of CD8+ T cells in HCC mice. Further studies reveal that the cyclin-dependent kinase 4 is deactivated by superoxide anion, and then cause the up-regulation of PD-L1. Our work provides molecular insights into HSC-mediated immune evasion of HCC, which may represent potential targets for HCC immunotherapy.
HostingRepositoryiProX
AnnounceDate2024-04-08
AnnouncementXMLSubmission_2024-04-10_01:36:12.627.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBo Tang
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-10 01:35:48ID requested
12024-04-10 01:36:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: superoxide anion, fluorescence imaging, immune evasion, hepatic stellate cells
Contact List
Bo Tang
contact affiliationCollege of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Shandong Normal University
contact emailtangb@sdnu.edu.cn
lab head
Bo Tang
contact affiliationCollege of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Shandong Normal University
contact emailtangb@sdnu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI