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PXD051316-1

PXD051316 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHDAC4 interactors identified by MS/MS
DescriptionAccess to DNA is the first level of control in regulating gene transcription, a control that is also critical for maintaining DNA integrity. Cellular senescence is characterized by profound transcriptional rearrangements and accumulation of DNA lesions. Here, we discovered an epigenetic complex between HDAC4 and HDAC1/HDAC2 that is involved in the erase of H2BK120 acetylation. The HDAC4/HDAC1/HDAC2 complex modulates the efficiency of DNA repair by homologous recombination, through dynamic deacetylation of H2BK120. Deficiency of HDAC4 leads to accumulation of H2BK120ac, impaired recruitment of BRCA1 and CtIP to the site of lesions, accumulation of damaged DNA and senescence. In senescent cells this complex is disassembled because of increased proteasomal degradation of HDAC4. Forced expression of HDAC4 during RAS-induced senescence reduces the genomic spread of γH2AX and affects H2BK120ac levels, which are increased in DNA-damaged regions accumulated during RAS-induced senescence. In summary, degradation of HDAC4 during senescence causes the accumulation of damaged DNA and contributes to the activation of the transcriptional program controlled by super-enhancers that maintains senescence.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_05:10:17.436.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD051316
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterEros DIGIORGIO
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLCQ Classic
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-09 11:44:45ID requested
12024-10-17 05:10:18announced
Publication List
10.6019/PXD051316;
10.1093/nar/gkae501;
Di Giorgio E, Dalla E, Tolotto V, D'Este F, Paluvai H, Ranzino L, Brancolini C, HDAC4 influences the DNA damage response and counteracts senescence by assembling with HDAC1/HDAC2 to control H2BK120 acetylation and homology-directed repair. Nucleic Acids Res, 52(14):8218-8240(2024) [pubmed]
Keyword List
submitter keyword: HDAC4
senescence
MS/MS
Contact List
Eros Di Giorgio
contact affiliationUniversity of Udine
contact emaileros.digiorgio@uniud.it
lab head
Eros DIGIORGIO
contact affiliationUNIUD
contact emaileros87.digiorgio@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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