PXD051316-1

PXD051316 is an original dataset announced via ProteomeXchange.

Title	HDAC4 interactors identified by MS/MS
Description	Access to DNA is the first level of control in regulating gene transcription, a control that is also critical for maintaining DNA integrity. Cellular senescence is characterized by profound transcriptional rearrangements and accumulation of DNA lesions. Here, we discovered an epigenetic complex between HDAC4 and HDAC1/HDAC2 that is involved in the erase of H2BK120 acetylation. The HDAC4/HDAC1/HDAC2 complex modulates the efficiency of DNA repair by homologous recombination, through dynamic deacetylation of H2BK120. Deficiency of HDAC4 leads to accumulation of H2BK120ac, impaired recruitment of BRCA1 and CtIP to the site of lesions, accumulation of damaged DNA and senescence. In senescent cells this complex is disassembled because of increased proteasomal degradation of HDAC4. Forced expression of HDAC4 during RAS-induced senescence reduces the genomic spread of γH2AX and affects H2BK120ac levels, which are increased in DNA-damaged regions accumulated during RAS-induced senescence. In summary, degradation of HDAC4 during senescence causes the accumulation of damaged DNA and contributes to the activation of the transcriptional program controlled by super-enhancers that maintains senescence.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:10:17.436.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051316
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eros DIGIORGIO
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-09 11:44:45	ID requested
⏵ 1	2024-10-17 05:10:18	announced

10.6019/PXD051316;

10.1093/nar/gkae501;

Di Giorgio E, Dalla E, Tolotto V, D'Este F, Paluvai H, Ranzino L, Brancolini C, HDAC4 influences the DNA damage response and counteracts senescence by assembling with HDAC1/HDAC2 to control H2BK120 acetylation and homology-directed repair. Nucleic Acids Res, 52(14):8218-8240(2024) [pubmed]

submitter keyword: HDAC4

senescence

MS/MS

Eros Di Giorgio
contact affiliation	University of Udine
contact email	eros.digiorgio@uniud.it
lab head
Eros DIGIORGIO
contact affiliation	UNIUD
contact email	eros87.digiorgio@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD051316

PRIDE project URI

• PRIDE
  1. PXD051316
     1. Label: PRIDE project
     2. Name: HDAC4 interactors identified by MS/MS