PXD051303 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | eIF5A-dependent feedback inhibition from mRNA translation elongation to initiation induces apoptosis in tumour cells |
| Description | Cancer progression is associated with disruption in protein synthesis, and novel therapies are being developed to target specific aspects of this process. Traditionally, these treatments have concentrated on the initiation phase of mRNA translation. However, recent discoveries indicate that issues in the elongation phase of translation, particularly alterations in eEF2 and eIF5A, also contribute to cancer cell growth. This research delves into the role of eIF5A, a crucial participant in this process, and its impact on mRNA translation elongation and initiation, as well as the subsequent changes in the nascent proteome. To investigate this, we utilize dynamic SILAC in conjunction with GC7 at both early and late treatment stages. By comprehending the state of translation after eIF5A inhibition, we can distinguish between direct and indirect protein targets of eIF5A. This understanding paves the way for more precise strategies in therapeutic efforts aimed at regulating mRNA translation and protein synthesis. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-05 |
| AnnouncementXML | Submission_2025-11-05_09:32:13.762.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sandip Kumar Patel |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-04-09 05:29:29 | ID requested | |
| ⏵ 1 | 2025-11-05 09:32:14 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: eEF2, Elongation, GC7, DHPS, mRNA Translation control, Protein Synthesis, ISRIB, DOHH, Initiation, Hypusination, eIF2,eIF5A |
Contact List
| Dr Anne E Willis |
|---|
| contact affiliation | MRC Toxicology Unit, Gleeson Building, Tennis Court Road, University of Cambridge, CB2 1QR |
| contact email | tls36@cam.ac.uk |
| lab head | |
| Sandip Kumar Patel |
|---|
| contact affiliation | Proteomics Core Facility, MRC Toxicology Unit, University of Cambridge |
| contact email | proteomics@mrc-tox.cam.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051303
- Label: PRIDE project
- Name: eIF5A-dependent feedback inhibition from mRNA translation elongation to initiation induces apoptosis in tumour cells
