PXD051274-1

PXD051274 is an original dataset announced via ProteomeXchange.

Title	Liver sinusoidal endothelial cells constitute a major route for hemoglobin clearance
Description	Mild hemolysis of senescent erythrocytes occurs physiologically in the spleen resulting in Hb release, whereas pathologic erythrocyte rupture characterizes several diseases. Iron recycling from Hb and Hb detoxification have been attributed to the sequestration of Hb-haptoglobin complexes by macrophages. However, it remained unclear whether other efficient routes of Hb clearance exist. We identified liver sinusoidal endothelial cells (LSECs) as the primary cells responsible for Hb uptake, a process that involves macropinocytosis and operates independently of the Hb-haptoglobin receptor CD163. To further study the functional adaptation of LSECs for Hb clearance, we performed two label-free proteomic analyses: i) using young BALB/C female mice, we compared the proteomes of the following cell types: LSECs, liver macrophages (Kupffer cells), splenic red pulp macrophages, and endothelial cells from the spleen and the heart (set of 15 samples, 5 sample groups, three replicates; ii) we determined changes of protein levels in LSECs after stressed erythrocyte injection in C57BL/6J female mice (set of 10 samples, 2 sample groups, five replicates). In the former analysis, we found that LSECs show high protein levels of enzymes of the heme-catabolic pathway, ferritins, and macropinocytosis regulators. In the later experiment, we identified Hb subunits as the top-increased proteins in LSECs upon erythrocyte injection in mice.
HostingRepository	PRIDE
AnnounceDate	2025-11-12
AnnouncementXML	Submission_2025-11-12_05:35:05.124.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-08 07:40:15	ID requested
⏵ 1	2025-11-12 05:35:05	announced

Dataset with its publication pending

submitter keyword: mouse, liver sinusoidal endothelial cells, hemoglobin clearance

Katarzyna Mleczko-Sanecka
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw, Warsaw, Poland
contact email	kmsanecka@iimcb.gov.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051274
     1. Label: PRIDE project
     2. Name: Liver sinusoidal endothelial cells constitute a major route for hemoglobin clearance