PXD051261-1

PXD051261 is an original dataset announced via ProteomeXchange.

Title	AE-MS to profile SLO-human plasma protein interactome landscape; HDX-MS and XL-MS to study protein interaction and dynamics of PLG-SLO
Description	Group A Streptococcus is known to exploit the human fibrinolysis system, particularly by targeting the plasminogen/plasmin pathway through redundant pathomechanisms. In this study, we began with affinity-enrichment mass spectrometry to map the interaction landscape between streptolysin O and human plasma, identifying human plasminogen as a specific binder to the streptolysin O. Subsequent biochemical assays validated this direct interaction and demonstrated the streptolysin O's role in enhancing tPA-mediated plasmin production. We then employed HDX-MS and XL-MS to further explore and characterize the molecular details of the plasminogen-streptolysin O complex formation, shedding light on its biological significance.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_00:29:43.212.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Di Tang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Streptococcus pyogenes MGAS315; NCBI TaxID: 198466;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Q Exactive HF-X; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-07 22:56:46	ID requested
⏵ 1	2024-10-17 00:29:43	announced

Dataset with its publication pending

submitter keyword: AE-MS, human, streptolysin O,Streptococcus pyogenes, XL-MS, HDX-MS, plasminogen, LC-MS/MS

Johan Malmström
contact affiliation	Division of Infection Medicine, Department of Clinical Sciences Lund, Faculty of Medicine, Lund University, Sweden
contact email	johan.malmstrom@med.lu.se
lab head
Di Tang
contact affiliation	Lund University
contact email	di.tang@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD051261

PRIDE project URI

• PRIDE
  1. PXD051261
     1. Label: PRIDE project
     2. Name: AE-MS to profile SLO-human plasma protein interactome landscape; HDX-MS and XL-MS to study protein interaction and dynamics of PLG-SLO