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PXD051261-1

PXD051261 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAE-MS to profile SLO-human plasma protein interactome landscape; HDX-MS and XL-MS to study protein interaction and dynamics of PLG-SLO
DescriptionGroup A Streptococcus is known to exploit the human fibrinolysis system, particularly by targeting the plasminogen/plasmin pathway through redundant pathomechanisms. In this study, we began with affinity-enrichment mass spectrometry to map the interaction landscape between streptolysin O and human plasma, identifying human plasminogen as a specific binder to the streptolysin O. Subsequent biochemical assays validated this direct interaction and demonstrated the streptolysin O's role in enhancing tPA-mediated plasmin production. We then employed HDX-MS and XL-MS to further explore and characterize the molecular details of the plasminogen-streptolysin O complex formation, shedding light on its biological significance.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_00:29:43.212.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDi Tang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Streptococcus pyogenes MGAS315; NCBI TaxID: 198466;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse; Q Exactive HF-X; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-07 22:56:46ID requested
12024-10-17 00:29:43announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: AE-MS, human, streptolysin O,Streptococcus pyogenes, XL-MS, HDX-MS, plasminogen, LC-MS/MS
Contact List
Johan Malmström
contact affiliationDivision of Infection Medicine, Department of Clinical Sciences Lund, Faculty of Medicine, Lund University, Sweden
contact emailjohan.malmstrom@med.lu.se
lab head
Di Tang
contact affiliationLund University
contact emaildi.tang@med.lu.se
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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