PXD051260-1

PXD051260 is an original dataset announced via ProteomeXchange.

Title	A non-canonical Arp2/3 complex is essential for Plasmodium DNA segregation and transmission of malaria.
Description	The malaria-causing parasite Plasmodium has a complex life cycle involving both vertebrate and mosquito hosts. Sexual stages or gametocytes are the only stage competent for mosquito transmission. Gametogenesis of the male gametocyte requires rapid rounds of genome replication to form flagellated gametes. Here, we describe the discovery of a non-canonical Plasmodium actin-related protein 2/3 (Arp2/3) complex essential for DNA segregation during male gametogenesis. Plasmodium Arp2/3 dynamically localizes within the nucleus to the endomitotic spindle and interacts with a kinetochore protein. Deletion of key Arp2/3 subunits or interfering with actin polymerisation leads to the formation of sub-haploid male gametes and a complete block in transmission through delayed developmental arrest at the oocyst stage. Our work identified an evolutionary divergent protein complex in malaria parasites that offers potential targets for transmission-blocking interventions.
HostingRepository	PRIDE
AnnounceDate	2025-04-22
AnnouncementXML	Submission_2025-04-22_05:22:10.376.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcin Luzarowski
SpeciesList	scientific name: Plasmodium berghei; NCBI TaxID: 5821;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-07 22:28:29	ID requested
⏵ 1	2025-04-22 05:22:10	announced

Dataset with its publication pending

submitter keyword: Arp2/3 complex, chromosome segregation,Malaria, Plasmodium

Franziska Hentzschel
contact affiliation	Integrative Parasitology, Centre for Infectious Diseases, University Hospital Heidelberg, Heidelberg University Medical Faculty; Heidelberg, Germany
contact email	franziska.hentzschel@med.uni-heidelberg.de
lab head
Marcin Luzarowski
contact affiliation	University of Heidelberg
contact email	m.luzarowski@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD051260

PRIDE project URI

• PRIDE
  1. PXD051260
     1. Label: PRIDE project
     2. Name: A non-canonical Arp2/3 complex is essential for Plasmodium DNA segregation and transmission of malaria.