PXD051216-1

PXD051216 is an original dataset announced via ProteomeXchange.

Title	Deciphering hepatitis B virus phospho-signature in primary human hepatocytes
Description	Phosphorylation is a major post-translation modification (PTM) of proteins, and small molecules, which is finely tuned by the activity of several hundred kinases and phosphatases. It controls most if not all cellular pathways including anti-viral responses. Accordingly, viruses often induce important changes in the phosphorylation of host factors that can either help or counteract viral replication. Surprisingly, among more than 500 kinases constituting the human kinome only few have been described as important for the Hepatitis B virus (HBV) infectious cycle, and most of them intervene during early or late infectious steps by phosphorylating the viral Core protein (HBc) protein. In addition, scarce information is available on the consequences of HBV infection on the activity of cellular kinases. The objective of this study was to investigate the global impact of HBV infection on the cellular phosphorylation landscape at early after infection. For this, primary human hepatocytes (PHH) were challenged or not with HBV, and a mass spectrometry (MS)-based quantitative phospho-proteomic analysis was conducted two- and seven-days post-infection.
HostingRepository	PRIDE
AnnounceDate	2024-05-02
AnnouncementXML	Submission_2024-05-02_05:23:31.917.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Hepatitis B virus subtype ayw; NCBI TaxID: 10418; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-05 07:14:26	ID requested
⏵ 1	2024-05-02 05:23:32	announced
2	2024-10-22 06:37:57	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: phosphorylation,Hepatitis B virus, nanoLC-MS/MS, primary hepatocytes

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051216
     1. Label: PRIDE project
     2. Name: Deciphering hepatitis B virus phospho-signature in primary human hepatocytes