PXD051211-2

PXD051211 is an original dataset announced via ProteomeXchange.

Title	R-protein composition of budding yeast PTC deficient 80S ribosomes
Description	Ribosomal RNA modifications are introduced during ribosome assembly by snoRNA and stand-alone modification enzyme based mechanisms in budding yeast. Lack of individual modifications in rRNA has a minor effect on yeast growth, ribosome biogenesis, and translation, which has complicated the definition of the biological function of rRNA modifications. We have constructed a S.cerevisiae strain lacking 7 25S rRNA modifications (Ψ2826, Um2921, Gm2619, m5C2870, Ψ2880, Um2724 and Gm2922)in peptidyl-transferase center. This strain exhibits severely compromised growth at 30°C, reduced level of global translation and actively translating ribosomes and cold sensitivity. Ribosomal protein composition of the mature 80S ribosomes was analyzed by SILAC based qMS approach. We have found that absence of PTC modifications around PTC does not affect significantly r-protein content of 80S ribosomes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:46:51.721.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Margus Leppik
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C; 6x(13)C; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-05 03:41:59	ID requested
1	2024-10-07 13:52:39	announced
⏵ 2	2024-10-22 06:46:53	announced	2024-10-22: Updated project metadata.

10.3390/ijms25105194;

Leppik M, Pomerants L, P, õ, ldes A, Mihkelson P, Remme J, Tamm T, Loss of Conserved rRNA Modifications in the Peptidyl Transferase Center Leads to Diminished Protein Synthesis and Cell Growth in Budding Yeast. Int J Mol Sci, 25(10):(2024) [pubmed]

submitter keyword: rRNA modifications.,S.cerevisiae

Tiina Tamm
contact affiliation	Chair of Molecular Biology Institute of Cellular and Molecular Biology Estonia (lab head)
contact email	ttamm@ut.ee
lab head
Margus Leppik
contact affiliation	University of Tartu
contact email	margus.leppik@ut.ee
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD051211

PRIDE project URI

• PRIDE
  1. PXD051211
     1. Label: PRIDE project
     2. Name: R-protein composition of budding yeast PTC deficient 80S ribosomes