PXD051201-1

PXD051201 is an original dataset announced via ProteomeXchange.

Title	Dissecting Sexual Dimorphism in Aortic Valve Stenosis by Proteomics
Description	Managing aortic valve stenosis (AVS) is challenging because of the lack of pharmacotherapies to halt/revert stenosis, deeming aortic valve replacement (AVR) the only effective treatment. AVS management is further confronted with staggering sexual dimorphism; women display more extensive fibrosis, while men present remarkably higher valve calcification. To accelerate the development of effective and sex-personalised pharmacotherapies, deeper molecular insights are needed. Hence, we aimed to characterise AVS sexual dimorphism using proteomics. Aortic valves were obtained from surgical AVR. Disease severity was assessed by echocardiography. Fifty valves (50% women) were homogenised, and the proteins were quantified by LC-MS/MS. The relevance of differentially expressed proteins (DEPs) in sexual dimorphism was appraised using protein-protein interaction (PPI) and functional enrichment analyses (FEA). DEPs were validated using immunohistochemistry, qRT-PCR and ELISA, with the aid of 30 additional independent valves.
HostingRepository	PRIDE
AnnounceDate	2025-10-13
AnnouncementXML	Submission_2025-10-12_18:09:56.678.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Vitorino
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-04 12:43:18	ID requested
⏵ 1	2025-10-12 18:09:57	announced

10.1186/s12014-025-09549-1;

Grego A, Sousa-Mendes C, Martins D, Sousa C, Ferreira AF, Saraiva F, Alves I, Espadas G, Miranda I, Leite-Moreira A, Sabid, ó E, Barros AS, Gavina C, Vitorino R, Falc, ã, o-Pires I, Nogueira-Ferreira R, Trindade F, Dissecting sexual dimorphism in aortic valve stenosis by proteomics. Clin Proteomics, 22(1):34(2025) [pubmed]

submitter keyword: DIA, aortic valve,human, sex dimorphism, LC-MS/MS

Fabio Trindade
contact affiliation	Faculty of Medicine of the University of Porto Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal
contact email	ftrindade@med.up.pt
lab head
Rui Vitorino
contact affiliation	Universidade de Aveiro
contact email	rvitorino@ua.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD051201

PRIDE project URI

• PRIDE
  1. PXD051201
     1. Label: PRIDE project
     2. Name: Dissecting Sexual Dimorphism in Aortic Valve Stenosis by Proteomics