PXD051176-1

PXD051176 is an original dataset announced via ProteomeXchange.

Title	Impact of high fat diet and platelet activation on pre-metastatic niche formation
Description	Metastasis involves dynamic interactions between tumor cells and the tumor microenvironment. Obesity has emerged as a significant contributor to tumor progression, particularly in breast cancer (BC). This study explores the impact of obesity on BC metastasis, focusing on the role of platelets and the formation of premetastatic niches (PMN). We found that high fat diet (HFD) leads to a basal pre-activation of platelets in circulation and endothelial cells in the lungs promoting the formation of “obese PMNs”. We observed that Fibronectin (FN) expression is upregulated both in platelets and endothelial cells in the lung of HFD-fed mice. We found that HFD led to enhanced interaction between platelets, tumor cells and endothelial cells within the PMN, increasing tumor cell homing and metastasis. Importantly, therapeutic interventions such as anti-platelet antibody administration or dietary restriction showed reduced metastatic cell homing and outgrowth. Moreover, FN blocking reduced tumor cell interaction with endothelial cell in HFD conditions. Importantly, analysis of coagulation parameters in triple negative BC patients before neoadjuvant treatment showed that subjects with reduced partial thromboplastin had a significantly shorter time to relapse. These findings highlight the significance of obesity-related factors and platelet-mediated coagulation in metastasis, suggesting potential therapeutic interventions and prognostic markers for BC patients.
HostingRepository	PRIDE
AnnounceDate	2025-03-22
AnnouncementXML	Submission_2025-03-22_12:32:03.431.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pilar Ximenez-Embun
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-03 06:36:53	ID requested
⏵ 1	2025-03-22 12:32:03	announced

10.1038/S41467-025-57938-9;

submitter keyword: platelets, breast cancer , pre-metastatic niche, iTRAQ,High fat diet

Javier Munoz
contact affiliation	Head of Proteomics Unit Spanish National Research Center (CNIO) c/Melchor Fernandez Almagro, 3, 28029 Madrid. Spain
contact email	jmunozpe@cnio.es
lab head
Pilar Ximenez-Embun
contact affiliation	Proteomics Group
contact email	mpximenez@cnio.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051176
     1. Label: PRIDE project
     2. Name: Impact of high fat diet and platelet activation on pre-metastatic niche formation