PXD051171-1

PXD051171 is an original dataset announced via ProteomeXchange.

Title	GlycoIP: An Integrated Platform for the Simultaneous Profiling of Intact N- and O-glycopeptides from Human Semen
Description	Protein glycosylation in human semen is crucial for spermatogenesis, maturation, sperm motility, capacitation, and fertilization. However, the site-specific information on N- and O-glycosylation in human semen has not been fully and accurately identified, particularly O-glycosylation. To address this, an integrated platform (termed GlycoIP) was developed for the simultaneous profiling of intact N- and O-glycopeptides from human semen. Although characterizing intact glycopeptides presents a challenge, their analysis can provide rich information about both the glycans and glycosites simultaneously. In this study, a total of 1,833 unique intact N-glycopeptides and 720 unique intact O-glycopeptides were reported based on GlycoIP. Much deeper and more accurate site-specific N/O-glycosylation information was revealed in this study, including 438 O-glycosites from 148 distinctive O-glycoproteins that may play important roles in human semen composition and function. In summary, GlycoIP provided a potentially useful method for the systematically profiling protein N/O-glycosylation in a single experiment. This lays the foundation for the functional studies of glycoproteins in male infertility.
HostingRepository	iProX
AnnounceDate	2024-04-01
AnnouncementXML	Submission_2024-04-03_02:32:41.092.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	deaminated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-03 02:32:06	ID requested
⏵ 1	2024-04-03 02:32:41	announced

Dataset with its publication pending

submitter keyword: Semen, Sperm, N-glycosylation, O-glycosylation, Glycoproteomics, LC-MS/MS

Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
lab head
Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
dataset submitter

iProX dataset URI