PXD051136-1

PXD051136 is an original dataset announced via ProteomeXchange.

Title	SCAMP3 is a component of insulin granules in MIN6 -cells and regulates insulin secretion and content
Description	Pancreatic -cells in the islets of Langerhans are key to maintaining glucose homeostasis, by secreting the peptide hormone insulin. Insulin is packaged within vesicles named insulin secretory granules (ISGs), that have recently been considered to have intrinsic structures and proteins that regulate insulin granule maturation, trafficking, and secretion. Previously, studies have identified a handful of novel ISG-associated proteins using different separation techniques. Here, this study combines an optimized ISG isolation technique and mass spectrometry-based proteomics, with an unbiased and targeted machine learning approach to uncover 211 ISG-associated proteins. Five of these proteins have not been previously ISG-associated: Syntaxin-7, Synaptophysin, Synaptotagmin-13, Zinc transporter ZIP8 and Scamp3. We validate the role for one of these novel ISG-associated proteins (Scamp3) in regulating insulin storage and secretion from -cells for the first time. These data provide the basis for future investigation into beta cell biology and the regulation of insulin secretion.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_14:46:35.469.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Larance
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-01 17:29:42	ID requested
⏵ 1	2025-05-06 14:46:36	announced

10.2337/db24-0355;

Norris N, Yau B, Famularo C, Webster H, Loudovaris T, Thomas HE, Larance M, Senior AM, Kebede MA, Optimized Proteomic Analysis of Insulin Granules From MIN6 Cells Identifies Scamp3, a Novel Regulator of Insulin Secretion and Content. Diabetes, 73(12):2045-2054(2024) [pubmed]

submitter keyword: pancreas, beta cell,insulin, SCAMP3, islet

Mark Larance
contact affiliation	Charles Perkins Centre and School of Medical Sciences, University of Sydney, Sydney, Australia.
contact email	mark.larance@sydney.edu.au
lab head
Mark Larance
contact affiliation	The University of Sydney
contact email	mark.larance@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051136

PRIDE project URI

• PRIDE
  1. PXD051136
     1. Label: PRIDE project
     2. Name: SCAMP3 is a component of insulin granules in MIN6 -cells and regulates insulin secretion and content