PXD051100-1

PXD051100 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Coupland et al., Cryo-EM of synaptic vesicles reveals loading-induced V-ATPase dissociation
Description	This dataset consists of 4 raw mass spectrometry files and associated peak lists and results files, acquired on a Bruker timsTOF Pro 2 mass spectrometer operated in Data-Dependent Acquisition mode. Synaptic vesicle and cleared rat brain lysate samples were prepared and separated in SDS-PAGE gel, then processed for in-gel protease digestion by Claire Coupland. Mass spectrometric acquisition and database searches were performed by Network Biology Collaborative Centre at the Lunenfeld-Tanenbaum Research Institute. Mass spec results were analyzed by Ji-Young Youn and John Rubinstein. The files are associated with a manuscript by Coupland et al. that determines the structure of synaptic vesicle V-ATPases and their associated proteins using Cryo-EM structural analysis. Mass spectrometry analysis is used to determine the protein components in purified synaptic vesicles. John Rubinstein is the corresponding author for the manuscript and should be contacted for questions regarding this dataset (john.rubinstein@utoronto.ca).
HostingRepository	MassIVE
AnnounceDate	2024-06-06
AnnouncementXML	Submission_2024-06-06_08:33:02.244.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ji-Young Youn
SpeciesList	scientific name: rat; scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116;
ModificationList	N-acetylated residue; L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
Instrument	timsTOF Pro 2

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-30 05:48:27	ID requested
⏵ 1	2024-06-06 08:33:02	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Cryo electron microscopy, v-ATPase, synaptic vesicles

submitter keyword: Cryo electron microscopy, v-ATPase, synaptic vesicles

Contact List

John Rubinstein
contact affiliation	Molecular Medicine Program, The Hospital for Sick Children; Department of Biochemistry and Department of Medical Biophysics, The University of Toronto
contact email	john.rubinstein@utoronto.ca
lab head
Ji-Young Youn
contact affiliation	Hospital for Sick Children
contact email	jiyoung.youn@sickkids.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094439/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094439/