PXD051090-2

PXD051090 is an original dataset announced via ProteomeXchange.

Title	Human mitochondrial TACO1-FLAG SILAC AP-MS
Description	The prokaryotic translation elongation factor P (EF-P), and the eukaryotic/archeal counterparts eIF5A/aIF5A, are proteins that serve a crucial role in mitigating ribosomal stalling during the translation of specific sequences, notably those containing consecutive proline residues. Although mitochondrial DNA-encoded proteins synthesized by mitochondrial ribosomes also contain polyproline stretches, an EF-P/eIF5A mitochondrial counterpart remains unidentified. Here, we show that the missing factor is the translational activator of COX1 (TACO1), a protein causative of a juvenile form of neurodegenerative Leigh's syndrome associated with cytochrome c oxidase deficiency. By using a combination of metabolic labeling, puromycin release, and ribosome profiling experiments, we show that TACO1 is required for the rapid synthesis of the poly-proline rich COX1 and COX3 proteins, while its requirement is negligible for other proteins. In agreement with a role in translation rate regulation, we show that TACO1 cooperates with the N-terminal extension of the large ribosomal subunit bL27m to provide stability to the peptidyl-transferase center during elongation, and that excess TACO1 enhances overall translation rate. We conclude that TACO1 is a Translation Accelerator and propose it as a promising target to regulate mitochondrial protein synthesis in disease scenarios.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:57:45.089.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051090
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michele Brischigliaro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-29 07:36:50	ID requested
1	2024-09-06 09:02:05	announced
⏵ 2	2024-10-22 06:57:45	announced	2024-10-22: Updated project metadata.

10.1093/NAR/GKAE645;

10.6019/PXD051090;

submitter keyword: AP-MS, Mitochondria,TACO1, SILAC

Antoni Barrientos
contact affiliation	Univeristy of Miami Miller School of Medicine
contact email	ABarrientos@med.miami.edu
lab head
Michele Brischigliaro
contact affiliation	University of Miami Miller School of Medicine
contact email	mxb2708@miami.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051090

PRIDE project URI

• PRIDE
  1. PXD051090
     1. Label: PRIDE project
     2. Name: Human mitochondrial TACO1-FLAG SILAC AP-MS