PXD051082-1

PXD051082 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The Cryptosporidium signaling kinase CDPK5 plays an important role in male gametogenesis and parasite virulence
Description	The protozoan parasite Cryptosporidium is a leading cause of diarrhea associated mortality in young children. The parasite’s lifecycle involves a highly coordinated and timely progression from asexual proliferative to sexual stages leading to formation of the transmissible oocyst. Underlying molecular signaling mechanisms orchestrating sexual development are not known. Here, we describe the function of a signaling kinase in Cryptosporidium male gametogenesis. We reveal the expression of Cryptosporidium parvum calcium-dependent protein kinase 5 (CDPK5) during male gamete development, and its important role in egress of mature gametes. Genetic ablation of this kinase results in viable parasites, indicating that this gene is dispensable for parasite survival. Interestingly, cdpk5 deletion significantly decreases parasite virulence and impacts oocyst shedding in immunocompromised mice. Using phosphoproteomics, we identify possible substrates of this kinase. Collectively, these findings illuminate parasite cell biology by revealing an important mechanism controlling male gamete production and identifies a potential target to reduce within-host reinfection as well as block disease transmission.
HostingRepository	jPOST
AnnounceDate	2024-06-04
AnnouncementXML	Submission_2024-06-04_09:12:57.080.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Justine Arrington
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; deamidated L-glutamine; deamidated L-asparagine
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-28 21:37:24	ID requested
⏵ 1	2024-06-04 09:12:57	announced

Publication List

Nava MG, Szewczyk J, Arrington JV, Alam T, Vinayak S, The Cryptosporidium signaling kinase CDPK5 plays an important role in male gametogenesis and parasite virulence. Cell Rep, 43(6):114263(2024) [pubmed]

Nava MG, Szewczyk J, Arrington JV, Alam T, Vinayak S, The Cryptosporidium signaling kinase CDPK5 plays an important role in male gametogenesis and parasite virulence. Cell Rep, 43(6):114263(2024) [pubmed]

Keyword List

submitter keyword: Phosphoproteomics, kinase signaling, Cryptosporidium, LFQ

submitter keyword: Phosphoproteomics, kinase signaling, Cryptosporidium, LFQ

Contact List

Sumiti Vinayak
lab head
Justine Arrington
contact affiliation	University of Illinois at Urbana-Champaign
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003017/