PXD051075-1

PXD051075 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Conventional cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure
Description	We developed an approach to in situ XL-MS which utilizes pre-stabilization with formaldehyde prior to crosslinker introduction. Applications of this method can boost crosslinker signal and allows for membrane-impermeable reagents to be used for studying protein-interaction landscapes in intact human cells.
HostingRepository	PRIDE
AnnounceDate	2024-04-12
AnnouncementXML	Submission_2024-04-12_12:46:17.469.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Michael
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-28 18:43:02	ID requested
⏵ 1	2024-04-12 12:46:17	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: in situ XL-MS, formaldehyde fixaiton, crosslinking mass spectrometry

submitter keyword: in situ XL-MS, formaldehyde fixaiton, crosslinking mass spectrometry

Contact List

David C. Schriemer
contact affiliation	Department of Chemstry & Department of Biochemistry and Molecular Biology, University of Clagary, Canada
contact email	dschriem@ucalgary.ca
lab head
Andrew Michael
contact affiliation	University of Calgary
contact email	andrew.michael@ucalgary.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD051075
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD051075

PRIDE project URI

Repository Record List

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