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PXD050991-1

PXD050991 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome dynamics in iPSC-derived human dopaminergic neurons – part 1: comprehensive coverage of the proteome using DIA
DescriptionDopaminergic neurons participate in fundamental physiological processes and are the cell type primarily affected in Parkinson’s disease (PD). Their analysis is challenging due to the intricate nature of their function, their involvement in diverse neurological processes, their heterogeneity and localization in deep brain regions. Consequently, most of the research on the protein dynamics of dopaminergic neurons has been performed in animal cells ex vivo. Here we use iPSC-derived, human mid-brain specific dopaminergic neurons to study general features of their proteome biology. We cover the proteome to a depth of 9,409 proteins and use dynamic SILAC to measure the half-life of more than 4,300 proteins. Our study provides a workflow and resource for future applications of quantitative proteomics in iPSC-derived human neurons.
HostingRepositoryPRIDE
AnnounceDate2024-09-12
AnnouncementXMLSubmission_2024-09-12_02:03:16.517.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterClaudia Cavarischia Rega
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-26 09:48:44ID requested
12024-09-12 02:03:16announced
22024-10-22 06:58:06announced2024-10-22: Updated project metadata.
Publication List
10.1016/J.MCPRO.2024.100838;
Keyword List
submitter keyword: proteomics, DIA, iPSC, axon,dopaminergic neurons, protein turnover
Contact List
Boris Macek
contact affiliationProteome Center Tuebingen Institute for Cell Biology University of Tuebingen Auf der Morgenstelle 15 72076 Tuebingen
contact emailboris.macek@uni-tuebingen.de
lab head
Claudia Cavarischia Rega
contact affiliationProteome Center Tuebingen Interfaculty Institute for Cell Biology University of Tuebingen
contact emailclaudia.cavarischia-rega@uni-tuebingen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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